Mitochondrial DNA and Functional Investigations into the Radiosensitivity of Four Mouse Strains
Joint Authors
Tian, Yeping
Yang, Shanmin
Ma, Jun
Vidyasagar, Sadasivan
Zhang, Steven B.
Zhang, Lurong
Chen, Chun
Zhang, Amy
Guo, Yongson
Litvinchuk, Alexandra
Swarts, Steven
Yin, Liangjie
Zhang, Zhenhuan
Zhang, Mei
Okunieff, Paul
Maguire, David
Casey-Sawicki, Katherine
Han, Deping
Wang, Xiaohui
Source
International Journal of Cell Biology
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-12
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
We investigated whether genetic radiosensitivity-related changes in mtDNA/nDNA ratios are significant to mitochondrial function and if a material effect on mtDNA content and function exists.
BALB/c (radiosensitive), C57BL/6 (radioresistant), and F1 hybrid mouse strains were exposed to total body irradiation.
Hepatic genomic DNA was extracted, and mitochondria were isolated.
Mitochondrial oxygen consumption, ROS, and calcium-induced mitochondrial swelling were measured.
Radiation influenced strain-specific survival in vivo.
F1 hybrid survival was influenced by maternal input.
Changes in mitochondrial content corresponded to survival in vivo among the 4 strains.
Calcium-induced mitochondrial swelling was strain dependent.
Isolated mitochondria from BALB/c mice were significantly more sensitive to calcium overload than mitochondria from C57BL/6 mice.
Maternal input partially influenced the recovery effect of radiation on calcium-induced mitochondrial swelling in F1 hybrids; the hybrid with a radiosensitive maternal lineage exhibited a lower rate of recovery.
Hybrids had a survival rate that was biased toward maternal input.
mtDNA content and mitochondrial permeability transition pores (MPTP) measured in these strains before irradiation reflected a dominant input from the parent.
After irradiation, the MPTP opened sooner in radiosensitive and hybrid strains, likely triggering intrinsic apoptotic pathways.
These findings have important implications for translation into predictors of radiation sensitivity/resistance.
American Psychological Association (APA)
Zhang, Steven B.& Maguire, David& Zhang, Mei& Tian, Yeping& Yang, Shanmin& Zhang, Amy…[et al.]. 2014. Mitochondrial DNA and Functional Investigations into the Radiosensitivity of Four Mouse Strains. International Journal of Cell Biology،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-503212
Modern Language Association (MLA)
Zhang, Steven B.…[et al.]. Mitochondrial DNA and Functional Investigations into the Radiosensitivity of Four Mouse Strains. International Journal of Cell Biology No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-503212
American Medical Association (AMA)
Zhang, Steven B.& Maguire, David& Zhang, Mei& Tian, Yeping& Yang, Shanmin& Zhang, Amy…[et al.]. Mitochondrial DNA and Functional Investigations into the Radiosensitivity of Four Mouse Strains. International Journal of Cell Biology. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-503212
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-503212