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Identification of Cassava MicroRNAs under Abiotic Stress
Joint Authors
Rodríguez-Zapata, Fausto
Ayling, Sarah
Ballén-Taborda, Carolina
Tohme, Joe
Becerra Lopez-Lavalle, Luis Augusto
Plata, Germán
Duitama, Jorge
Source
International Journal of Genomics
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-14
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
The study of microRNAs (miRNAs) in plants has gained significant attention in recent years due to their regulatory role during development and in response to biotic and abiotic stresses.
Although cassava (Manihot esculenta Crantz) is tolerant to drought and other adverse conditions, most cassava miRNAs have been predicted using bioinformatics alone or through sequencing of plants challenged by biotic stress.
Here, we use high-throughput sequencing and different bioinformatics methods to identify potential cassava miRNAs expressed in different tissues subject to heat and drought conditions.
We identified 60 miRNAs conserved in other plant species and 821 potential cassava-specific miRNAs.
We also predicted 134 and 1002 potential target genes for these two sets of sequences.
Using real time PCR, we verified the condition-specific expression of 5 cassava small RNAs relative to a non-stress control.
We also found, using publicly available expression data, a significantly lower expression of the predicted target genes of conserved and nonconserved miRNAs under drought stress compared to other cassava genes.
Gene Ontology enrichment analysis along with condition specific expression of predicted miRNA targets, allowed us to identify several interesting miRNAs which may play a role in stress-induced posttranscriptional regulation in cassava and other plants.
American Psychological Association (APA)
Ballén-Taborda, Carolina& Plata, Germán& Ayling, Sarah& Rodríguez-Zapata, Fausto& Becerra Lopez-Lavalle, Luis Augusto& Duitama, Jorge…[et al.]. 2013. Identification of Cassava MicroRNAs under Abiotic Stress. International Journal of Genomics،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-503878
Modern Language Association (MLA)
Ballén-Taborda, Carolina…[et al.]. Identification of Cassava MicroRNAs under Abiotic Stress. International Journal of Genomics No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-503878
American Medical Association (AMA)
Ballén-Taborda, Carolina& Plata, Germán& Ayling, Sarah& Rodríguez-Zapata, Fausto& Becerra Lopez-Lavalle, Luis Augusto& Duitama, Jorge…[et al.]. Identification of Cassava MicroRNAs under Abiotic Stress. International Journal of Genomics. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-503878
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-503878