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Transient Limb Ischemia Alters Serum Protein Expression in Healthy Volunteers : Complement C3 and Vitronectin May Be Involved in Organ Protection Induced by Remote Ischemic Preconditioning
Joint Authors
Jin, San-Qing
Zhao, Yang
Pang, Ting
Pan, San-Qiang
Zhang, Nan-Rong
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-02
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
The protective mechanism underlying remote ischemic preconditioning (RIPC) is unclear.
This study aims to verify whether the protein expression profile in the serum could be altered by RIPC and to detect potential protein mediators.
Transient limb ischemia consisting of three cycles of 5-min ischemia followed by 5-min reperfusion was performed on sixty healthy volunteers.
Serum samples were collected at 30 min before transient limb ischemia and at 1 hour (h), 3 h, 8 h, 24 h, and 48 h after completion of three cycles.
Changes in the serum protein profile were analyzed by two-dimensional gel electrophoresis and proteins were identified by MALDI-TOF/TOF mass spectrometry.
Fourteen differentially expressed proteins were identified and, respectively, involved in immune system, lipid binding and metabolism, apoptosis, and blood coagulation.
Complement C3, vitronectin, and apolipoprotein A-I were further confirmed by western blotting, and the results showed that their contents decreased significantly after transient limb ischemia.
It is concluded that transient limb ischemia alters the serum protein expression profile in human being, and that reduction of serum contents of complement C3 and vitronectin may represent an important part of the mechanism whereby RIPC confers its protection.
American Psychological Association (APA)
Pang, Ting& Zhao, Yang& Zhang, Nan-Rong& Jin, San-Qing& Pan, San-Qiang. 2013. Transient Limb Ischemia Alters Serum Protein Expression in Healthy Volunteers : Complement C3 and Vitronectin May Be Involved in Organ Protection Induced by Remote Ischemic Preconditioning. Oxidative Medicine and Cellular Longevity،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-503960
Modern Language Association (MLA)
Pang, Ting…[et al.]. Transient Limb Ischemia Alters Serum Protein Expression in Healthy Volunteers : Complement C3 and Vitronectin May Be Involved in Organ Protection Induced by Remote Ischemic Preconditioning. Oxidative Medicine and Cellular Longevity No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-503960
American Medical Association (AMA)
Pang, Ting& Zhao, Yang& Zhang, Nan-Rong& Jin, San-Qing& Pan, San-Qiang. Transient Limb Ischemia Alters Serum Protein Expression in Healthy Volunteers : Complement C3 and Vitronectin May Be Involved in Organ Protection Induced by Remote Ischemic Preconditioning. Oxidative Medicine and Cellular Longevity. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-503960
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-503960