Study of Enzymatic Hydrolysis of Fructans from Agave salmiana Characterization and Kinetic Assessment
Joint Authors
Ruiz-Cabrera, Miguel
González-Chávez, Marco Martín
Moscosa-Santillán, Mario
Michel-Cuello, Christian
Grajales-Lagunes, Alicia
Ortiz-Cerda, Imelda
Moreno-Vilet, Lorena
Bonnin, Johanne
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-02
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Information Technology and Computer Science
Abstract EN
Fructans were extracted from Agave salmiana juice, characterized and subjected to hydrolysis process using a commercial inulinase preparation acting freely.
To compare the performance of the enzymatic preparation, a batch of experiments were also conducted with chicory inulin (reference).
Hydrolysis was performed for 6 h at two temperatures (50, 60∘C) and two substrate concentrations (40, 60 mg/ml).
Hydrolysis process was monitored by measuring the sugars released and residual substrate by HPLC.
A mathematical model which describes the kinetics of substrate degradation as well as fructose production was proposed to analyze the hydrolysis assessment.
It was found that kinetics were significantly influenced by temperature, substrate concentration, and type of substrate (P<0.01).
The extent of substrate hydrolysis varied from 82 to 99%.
Hydrolysis product was mainly constituted of fructose, obtaining from 77 to 96.4% of total reducing sugars.
American Psychological Association (APA)
Michel-Cuello, Christian& Ortiz-Cerda, Imelda& Moreno-Vilet, Lorena& Grajales-Lagunes, Alicia& Moscosa-Santillán, Mario& Bonnin, Johanne…[et al.]. 2012. Study of Enzymatic Hydrolysis of Fructans from Agave salmiana Characterization and Kinetic Assessment. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-504381
Modern Language Association (MLA)
Michel-Cuello, Christian…[et al.]. Study of Enzymatic Hydrolysis of Fructans from Agave salmiana Characterization and Kinetic Assessment. The Scientific World Journal No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-504381
American Medical Association (AMA)
Michel-Cuello, Christian& Ortiz-Cerda, Imelda& Moreno-Vilet, Lorena& Grajales-Lagunes, Alicia& Moscosa-Santillán, Mario& Bonnin, Johanne…[et al.]. Study of Enzymatic Hydrolysis of Fructans from Agave salmiana Characterization and Kinetic Assessment. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-504381
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-504381