Effects of Tannin Extract from Gongronema latifolium Leaves on Lipoxygenase Cucumeropsis manii Seeds
Joint Authors
Eze, Sabinus O. O.
Nwanguma, Bennett C.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-19
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Lipoxygenase (EC 1.13.11.12) was partially purified from germinated seeds of Cucumeropsis manii to a purification fold of 47.14, enzyme activity recovery of 72.18%, and specific activity of 326.25 units/mg protein, using a three-step process of centrifugation, ammonium sulphate precipitation and gel filtration.
Kinetic properties show maximum activity at pH 6.0 and at optimum temperature of 40°C.
Inhibitory effects of the extract from Gongronema latifolium and two other known antioxidants: ascorbic acid and propyl gallate on lipoxygenase from Cucumeropsis manii were studied.
Result shows presence of inhibition with IC50 of 4.2×10−3 ± 0.09×10−3 g/L, 4.3×10−2± 0.11×10−2 g/L and 7.9×10−2 ± 0.11×10−2 g/L for the extract from ascorbic acid and propyl gallate, respectively.
The extract when compared to the other antioxidants exhibits a competitive mechanism of inhibition.
This tannin extract could be included during food processing as preservative against food deterioration that might be caused by oxidative enzymes such as lipoxygenase.
American Psychological Association (APA)
Eze, Sabinus O. O.& Nwanguma, Bennett C.. 2012. Effects of Tannin Extract from Gongronema latifolium Leaves on Lipoxygenase Cucumeropsis manii Seeds. Journal of Chemistry،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-504452
Modern Language Association (MLA)
Eze, Sabinus O. O.& Nwanguma, Bennett C.. Effects of Tannin Extract from Gongronema latifolium Leaves on Lipoxygenase Cucumeropsis manii Seeds. Journal of Chemistry No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-504452
American Medical Association (AMA)
Eze, Sabinus O. O.& Nwanguma, Bennett C.. Effects of Tannin Extract from Gongronema latifolium Leaves on Lipoxygenase Cucumeropsis manii Seeds. Journal of Chemistry. 2012. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-504452
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-504452