Ethanol Exposure Alters Protein Expression in a Mouse Model of Fetal Alcohol Spectrum Disorders
Joint Authors
Wang, Mu
Witzmann, Frank A.
Anthony, Bruce
You, Jin-Sam
Ringham, Heather N.
Zhou, Feng C.
Lai, Xianyin
Mason, Stephen
Source
International Journal of Proteomics
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-06-14
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Alcohol exposure during development can result in variable growth retardation and facial dysmorphology known as fetal alcohol spectrum disorders.
Although the mechanisms underlying the disorder are not fully understood, recent progress has been made that alcohol induces aberrant changes in gene expression and in the epigenome of embryos.
To inform the gene and epigenetic changes in alcohol-induced teratology, we used whole-embryo culture to identify the alcohol-signature protein profile of neurulating C6 mice.
Alcohol-treated and control cultures were homogenized, isoelectrically focused, and loaded for 2D gel electrophoresis.
Stained gels were cross matched with analytical software.
We identified 40 differentially expressed protein spots (P<0.01), and 9 spots were selected for LC/MS-MS identification.
Misregulated proteins include serotransferrin, triosephosphate isomerase and ubiquitin-conjugating enzyme E2 N.
Misregulation of serotransferrin and triosephosphate isomerase was confirmed with immunologic analysis.
Alteration of proteins with roles in cellular function, cell cycle, and the ubiquitin-proteasome pathway was induced by alcohol.
Several misregulated proteins interact with effectors of the NF-κB and Myc transcription factor cascades.
Using a whole-embryo culture, we have identified misregulated proteins known to be involved in nervous system development and function.
American Psychological Association (APA)
Mason, Stephen& Anthony, Bruce& Lai, Xianyin& Ringham, Heather N.& Wang, Mu& Witzmann, Frank A.…[et al.]. 2012. Ethanol Exposure Alters Protein Expression in a Mouse Model of Fetal Alcohol Spectrum Disorders. International Journal of Proteomics،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-504634
Modern Language Association (MLA)
Mason, Stephen…[et al.]. Ethanol Exposure Alters Protein Expression in a Mouse Model of Fetal Alcohol Spectrum Disorders. International Journal of Proteomics No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-504634
American Medical Association (AMA)
Mason, Stephen& Anthony, Bruce& Lai, Xianyin& Ringham, Heather N.& Wang, Mu& Witzmann, Frank A.…[et al.]. Ethanol Exposure Alters Protein Expression in a Mouse Model of Fetal Alcohol Spectrum Disorders. International Journal of Proteomics. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-504634
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-504634