Ethanol Exposure Alters Protein Expression in a Mouse Model of Fetal Alcohol Spectrum Disorders

Joint Authors

Wang, Mu
Witzmann, Frank A.
Anthony, Bruce
You, Jin-Sam
Ringham, Heather N.
Zhou, Feng C.
Lai, Xianyin
Mason, Stephen

Source

International Journal of Proteomics

Issue

Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2012-06-14

Country of Publication

Egypt

No. of Pages

10

Main Subjects

Natural & Life Sciences (Multidisciplinary)
Biology

Abstract EN

Alcohol exposure during development can result in variable growth retardation and facial dysmorphology known as fetal alcohol spectrum disorders.

Although the mechanisms underlying the disorder are not fully understood, recent progress has been made that alcohol induces aberrant changes in gene expression and in the epigenome of embryos.

To inform the gene and epigenetic changes in alcohol-induced teratology, we used whole-embryo culture to identify the alcohol-signature protein profile of neurulating C6 mice.

Alcohol-treated and control cultures were homogenized, isoelectrically focused, and loaded for 2D gel electrophoresis.

Stained gels were cross matched with analytical software.

We identified 40 differentially expressed protein spots (P<0.01), and 9 spots were selected for LC/MS-MS identification.

Misregulated proteins include serotransferrin, triosephosphate isomerase and ubiquitin-conjugating enzyme E2 N.

Misregulation of serotransferrin and triosephosphate isomerase was confirmed with immunologic analysis.

Alteration of proteins with roles in cellular function, cell cycle, and the ubiquitin-proteasome pathway was induced by alcohol.

Several misregulated proteins interact with effectors of the NF-κB and Myc transcription factor cascades.

Using a whole-embryo culture, we have identified misregulated proteins known to be involved in nervous system development and function.

American Psychological Association (APA)

Mason, Stephen& Anthony, Bruce& Lai, Xianyin& Ringham, Heather N.& Wang, Mu& Witzmann, Frank A.…[et al.]. 2012. Ethanol Exposure Alters Protein Expression in a Mouse Model of Fetal Alcohol Spectrum Disorders. International Journal of Proteomics،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-504634

Modern Language Association (MLA)

Mason, Stephen…[et al.]. Ethanol Exposure Alters Protein Expression in a Mouse Model of Fetal Alcohol Spectrum Disorders. International Journal of Proteomics No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-504634

American Medical Association (AMA)

Mason, Stephen& Anthony, Bruce& Lai, Xianyin& Ringham, Heather N.& Wang, Mu& Witzmann, Frank A.…[et al.]. Ethanol Exposure Alters Protein Expression in a Mouse Model of Fetal Alcohol Spectrum Disorders. International Journal of Proteomics. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-504634

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-504634