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Brazilian Propolis Suppresses Angiogenesis by Inducing Apoptosis in Tube-Forming Endothelial Cells through Inactivation of Survival Signal ERK12
Joint Authors
Eguchi, Ryoji
Fujimori, Yoshihiro
Kumazawa, Shigenori
Nakayama, Tsutomu
Kobayashi, Tomomi
Kunimasa, Kazuhiro
Kaji, Kazuhiko
Ahn, Mok-Ryeon
Nakano, Takashi
Ohta, Toshiro
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-10-31
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
We recently reported that propolis suppresses tumor-induced angiogenesis through tube formation inhibition and apoptosis induction in endothelial cells.
However, molecular mechanisms underlying such angiogenesis suppression by propolis have not been fully elucidated.
The aim of this study was to investigate the effects of ethanol extract of Brazilian propolis (EEBP) on two major survival signals, extracellular signal-regulated kinase 1/2 (ERK1/2) and Akt, and to elucidate whether changes in these signals were actually involved in antiangiogenic effects of the propolis.
Detection by western blotting revealed that EEBP suppressed phosphorylation of ERK1/2, but not that of Akt.
Pharmacological inhibition by U0126 demonstrated that ERK1/2 inactivation alone was enough to inhibit tube formation and induce apoptosis.
It was also shown that EEBP and U0126 similarly induced activation of caspase-3 and cleavage of poly ADP-ribose polymerase (PARP) and lamin A/C, all of which are molecular markers of apoptosis.
These results indicate that inhibition of survival signal ERK1/2, and subsequent induction of apoptosis, is a critical mechanism of angiogenesis suppression by EEBP.
American Psychological Association (APA)
Kunimasa, Kazuhiro& Ahn, Mok-Ryeon& Kobayashi, Tomomi& Eguchi, Ryoji& Kumazawa, Shigenori& Fujimori, Yoshihiro…[et al.]. 2010. Brazilian Propolis Suppresses Angiogenesis by Inducing Apoptosis in Tube-Forming Endothelial Cells through Inactivation of Survival Signal ERK12. Evidence-Based Complementary and Alternative Medicine،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-504941
Modern Language Association (MLA)
Kunimasa, Kazuhiro…[et al.]. Brazilian Propolis Suppresses Angiogenesis by Inducing Apoptosis in Tube-Forming Endothelial Cells through Inactivation of Survival Signal ERK12. Evidence-Based Complementary and Alternative Medicine No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-504941
American Medical Association (AMA)
Kunimasa, Kazuhiro& Ahn, Mok-Ryeon& Kobayashi, Tomomi& Eguchi, Ryoji& Kumazawa, Shigenori& Fujimori, Yoshihiro…[et al.]. Brazilian Propolis Suppresses Angiogenesis by Inducing Apoptosis in Tube-Forming Endothelial Cells through Inactivation of Survival Signal ERK12. Evidence-Based Complementary and Alternative Medicine. 2010. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-504941
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-504941