Obesity-Related Metabolomic Analysis of Human Subjects in Black Soybean Peptide Intervention Study by Ultraperformance Liquid Chromatography and Quadrupole-Time-of-Flight Mass Spectrometry
Joint Authors
Kwon, Dae Young
Kim, Min Jung
Yang, Hye Jeong
Kim, Jin Hee
Lee, Jong Ho
Kim, Kang Sung
Ahn, Chang-Won
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-04
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The present study aimed to identify key metabolites related to weight reduction in humans by studying the metabolic profiles of sera obtained from 34 participants who underwent dietary intervention with black soybean peptides (BSP) for 12 weeks.
This research is a sequel to our previous work in which the effects of BSP on BMI and blood composition of lipid were investigated.
Sera of the study were subjected to ultra performance liquid chromatography and quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS), and the data were analyzed using partial least-squares discriminate analysis (PLS-DA) score plots.
Body mass index and percent body fat of the test group were reduced.
Levels of betaine, benzoic acid, pyroglutamic acid, pipecolic acid, N-phenylacetamide, uric acid, l-aspartyl-l-phenylalanine, and lysophosphatidyl cholines (lysoPCs) (C18:1, C18:2, C20:1, and C20:4) showed significant increases.
Levels of l-proline, valine, l-leucine/isoleucine, hypoxanthine, glutamine, l-methionine, phenylpyruvic acid, several carnitine derivatives, and lysoPCs (C14:0, PC16:0, C15:0, C16:0, C17:1, C18:0, and C22:0) were significantly decreased.
In particular, lysoPC 16:0 with a VIP value of 12.02 is esteemed to be the most important metabolite for evaluating the differences between the 2 serum samples.
Our result confirmed weight-lowering effects of BSP, accompanied by favorable changes in metabolites in the subjects’ blood.
Therefore, this research enables us to better understand obesity and increases the predictability of the obesity-related risk by studying metabolites present in the blood.
American Psychological Association (APA)
Kim, Min Jung& Yang, Hye Jeong& Kim, Jin Hee& Ahn, Chang-Won& Lee, Jong Ho& Kim, Kang Sung…[et al.]. 2013. Obesity-Related Metabolomic Analysis of Human Subjects in Black Soybean Peptide Intervention Study by Ultraperformance Liquid Chromatography and Quadrupole-Time-of-Flight Mass Spectrometry. Journal of Obesity،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-505342
Modern Language Association (MLA)
Kim, Min Jung…[et al.]. Obesity-Related Metabolomic Analysis of Human Subjects in Black Soybean Peptide Intervention Study by Ultraperformance Liquid Chromatography and Quadrupole-Time-of-Flight Mass Spectrometry. Journal of Obesity No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-505342
American Medical Association (AMA)
Kim, Min Jung& Yang, Hye Jeong& Kim, Jin Hee& Ahn, Chang-Won& Lee, Jong Ho& Kim, Kang Sung…[et al.]. Obesity-Related Metabolomic Analysis of Human Subjects in Black Soybean Peptide Intervention Study by Ultraperformance Liquid Chromatography and Quadrupole-Time-of-Flight Mass Spectrometry. Journal of Obesity. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-505342
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-505342