Modulation of c-Fos and BDNF Protein Expression in Pentylenetetrazole-Kindled Mice following the Treatment with Novel Antiepileptic Compound HHL-6
Joint Authors
Siddiqui, Bina S.
Jawed, Huma
Ashraf, Nadeem
Malhi, Saima Mahmood
Hanif, Farina
Simjee, Shabana Usman
Kabir, Nurul
Begum, Sabira
Zubair, Farhat
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-29
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Brain-derived neurotrophic factor (BDNF) and c-Fos are shown to promote epileptogenesis and are taken as a marker of neuronal activity.
The present study investigated the expression of BDNF and c-Fos in mice brain with pentylenetetrazol- (PTZ-) induced generalized seizure and evaluated the effect of novel tryptamine derivative HHL-6 on the expression of these two markers.
The subconvulsive dose of PTZ (50 mg/kg) was administered on alternate days in the experimental groups until the seizure scores 4-5 developed in the PTZ-control group.
At the end of each experiment, animals were sacrificed, brain samples were collected and cryosectioned, and immunohistochemical analysis of BDNF and c-Fos protein was performed.
Data obtained from two sections per mouse (n=12 animals/group) is presented as means ± S.E.M.
The test compound HHL-6 demonstrated a potent anticonvulsant activity in the PTZ-induced seizure in mice.
Significant reduction in the BDNF (P<0.003) and c-Fos (P<0.01) protein expression was observed in the HHL-6 treated group.
Based on these results we suggest that one of the possible mechanisms of HHL-6 to inhibit epileptogenesis might be due to its controlling effect on the cellular and molecular expression of the factors that contribute to the development of epileptogenic plasticity in the CNS.
American Psychological Association (APA)
Malhi, Saima Mahmood& Jawed, Huma& Hanif, Farina& Ashraf, Nadeem& Zubair, Farhat& Siddiqui, Bina S.…[et al.]. 2014. Modulation of c-Fos and BDNF Protein Expression in Pentylenetetrazole-Kindled Mice following the Treatment with Novel Antiepileptic Compound HHL-6. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-505511
Modern Language Association (MLA)
Malhi, Saima Mahmood…[et al.]. Modulation of c-Fos and BDNF Protein Expression in Pentylenetetrazole-Kindled Mice following the Treatment with Novel Antiepileptic Compound HHL-6. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-505511
American Medical Association (AMA)
Malhi, Saima Mahmood& Jawed, Huma& Hanif, Farina& Ashraf, Nadeem& Zubair, Farhat& Siddiqui, Bina S.…[et al.]. Modulation of c-Fos and BDNF Protein Expression in Pentylenetetrazole-Kindled Mice following the Treatment with Novel Antiepileptic Compound HHL-6. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-505511
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-505511