Protective Effect of Alpha-Tocopherol Isomer from Vitamin E against the H2O2 Induced Toxicity on Dental Pulp Cells
Joint Authors
De Souza Costa, Carlos Alberto
Vargas, Fernanda da Silveira
Hebling, Josimeri
Soares, Diana Gabriela
Ribeiro, Ana Paula Dias
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-21
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
The aim of this study was to evaluate the protective effects of different concentrations of vitamin E alpha-tocopherol (α-T) isomer against the toxicity of hydrogen peroxide (H2O2) on dental pulp cells.
The cells (MDPC-23) were seeded in 96-well plates for 72 hours, followed by treatment with 1, 3, 5, or 10 mM α-T for 60 minutes.
They were then exposed or not to H2O2 for 30 minutes.
In positive and negative control groups, the cells were exposed to culture medium with or without H2O2 (0.018%), respectively.
Cell viability was evaluated by MTT assay (Kruskal-Wallis and Mann-Whitney tests; α=5%).
Significant reduction of cell viability (58.5%) was observed in positive control compared with the negative control.
Cells pretreated with α-T at 1, 3, 5, and 10 mM concentrations and exposed to H2O2 had their viability decreased by 43%, 32%, 25%, and 27.5%, respectively.
These values were significantly lower than those observed in the positive control, thereby showing a protective effect of α-T against the H2O2 toxicity.
Overall, the vitamin E α-T isomer protected the immortalized MDPC-23 pulp cells against the toxic effects of H2O2.
The most effective cell protection was provided by 5 and 10 mM concentrations of α-T.
American Psychological Association (APA)
Vargas, Fernanda da Silveira& Soares, Diana Gabriela& Ribeiro, Ana Paula Dias& Hebling, Josimeri& De Souza Costa, Carlos Alberto. 2014. Protective Effect of Alpha-Tocopherol Isomer from Vitamin E against the H2O2 Induced Toxicity on Dental Pulp Cells. BioMed Research International،Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-506162
Modern Language Association (MLA)
Vargas, Fernanda da Silveira…[et al.]. Protective Effect of Alpha-Tocopherol Isomer from Vitamin E against the H2O2 Induced Toxicity on Dental Pulp Cells. BioMed Research International No. 2014 (2014), pp.1-5.
https://search.emarefa.net/detail/BIM-506162
American Medical Association (AMA)
Vargas, Fernanda da Silveira& Soares, Diana Gabriela& Ribeiro, Ana Paula Dias& Hebling, Josimeri& De Souza Costa, Carlos Alberto. Protective Effect of Alpha-Tocopherol Isomer from Vitamin E against the H2O2 Induced Toxicity on Dental Pulp Cells. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-506162
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-506162