Coimmobilization of Naringinases on Silk Fibroin Nanoparticles and Its Application in Food Packaging
Joint Authors
Wu, Min-Hui
Zhou, Zhen-Zhen
Zhu, Lin
Zhang, Yu-Qing
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-01-14
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Engineering Sciences and Information Technology
Chemistry
Abstract EN
Bombyx mori silk fibroin is a macromolecular biopolymer with remarkable biocompatibility.
It was degummed and subjected to a series of treatments, including dissolution and dialysis, to yield an aqueous solution of silk fibroin, which was introduced rapidly into excess acetone to produce crystalline silk fibroin nanoparticles (SFNs), which were conjugated covalently with naringinase using glutaraldehyde as the cross-linking reagent.
The SFN naringinases are easily recovered by centrifugation and can be used repeatedly.
Naringinase is a bienzyme consisting of α-L-rhamnosidase and flavonoid-β-glucosidase.
The enzyme activity and its kinetics were similar to those of the native form, and the optimum reactive temperature for both is 55°C.
In our study, centrifugation allowed the separation of enzyme and substrate; after eight cycles the SFN naringinases retained >70% residual activity.
The highly efficient processing technology and the use of SFN as a novel vector for a bienzyme have great potential for research and the development of food processing such as the debittering of naringin-containing juices.
American Psychological Association (APA)
Wu, Min-Hui& Zhu, Lin& Zhou, Zhen-Zhen& Zhang, Yu-Qing. 2013. Coimmobilization of Naringinases on Silk Fibroin Nanoparticles and Its Application in Food Packaging. Journal of Nanoparticles،Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-506541
Modern Language Association (MLA)
Wu, Min-Hui…[et al.]. Coimmobilization of Naringinases on Silk Fibroin Nanoparticles and Its Application in Food Packaging. Journal of Nanoparticles No. 2013 (2013), pp.1-5.
https://search.emarefa.net/detail/BIM-506541
American Medical Association (AMA)
Wu, Min-Hui& Zhu, Lin& Zhou, Zhen-Zhen& Zhang, Yu-Qing. Coimmobilization of Naringinases on Silk Fibroin Nanoparticles and Its Application in Food Packaging. Journal of Nanoparticles. 2013. Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-506541
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-506541