Enzymatic Digestion and Selective Quantification of Underivatised Delta-9-Tetrahydrocannabinol and Cocaine in Human Hair Using Gas Chromatography-Mass Spectrometry
Joint Authors
Barker, James
Petróczi, Andrea
Breidi, Salah Eddine
Naughton, Declan P.
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-03
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Gas chromatography-mass spectrometric (GC-MS) methods for drug analysis routinely employ derivatising reagents.
The aim of this paper was to develop a method for the analysis of two recreational drugs, delta-9-tetrahydrocannabinol (Δ9-THC) and cocaine in hair samples using GC-MS, without prior derivatisation, thus allowing the sample to be reanalysed in its original form.
An enzymatic digestion technique was also developed.
Ten hair samples, that were known positive for either Δ9-THC and/or cocaine, were enzymatically digested, extracted, and then analysed by GC-MS.
All samples measured contained Δ9-THC and one sample contained cocaine.
The limits of detection (LOD) and quantification (LOQ) were 0.02 ng/mg and 0.05 ng/mg, respectively, for cocaine and 0.015 ng/mg and 0.02 ng/mg, respectively, for Δ9-THC.
The wide detection window, ease of direct analysis by GC-MS, lower detection limits of underivatised samples, and the stability of drugs using this technique may offer an improved method of analysis.
American Psychological Association (APA)
Breidi, Salah Eddine& Barker, James& Petróczi, Andrea& Naughton, Declan P.. 2012. Enzymatic Digestion and Selective Quantification of Underivatised Delta-9-Tetrahydrocannabinol and Cocaine in Human Hair Using Gas Chromatography-Mass Spectrometry. Journal of Analytical Methods in Chemistry،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-507171
Modern Language Association (MLA)
Breidi, Salah Eddine…[et al.]. Enzymatic Digestion and Selective Quantification of Underivatised Delta-9-Tetrahydrocannabinol and Cocaine in Human Hair Using Gas Chromatography-Mass Spectrometry. Journal of Analytical Methods in Chemistry No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-507171
American Medical Association (AMA)
Breidi, Salah Eddine& Barker, James& Petróczi, Andrea& Naughton, Declan P.. Enzymatic Digestion and Selective Quantification of Underivatised Delta-9-Tetrahydrocannabinol and Cocaine in Human Hair Using Gas Chromatography-Mass Spectrometry. Journal of Analytical Methods in Chemistry. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-507171
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-507171