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Flavonoids Identified from Korean Scutellaria baicalensis Georgi Inhibit Inflammatory Signaling by Suppressing Activation of NF-κB and MAPK in RAW 264.7 Cells
Joint Authors
Lee, Won-Sup
Lee, Ho-Jeong
Kim, Jin-A.
Yumnam, Silvia
Shin, Sung-Chul
Kim, Gon-Sup
Park, Hyeon-Soo
Hong, Gyeong-Eun
Kim, Eun-Hee
Nagappan, Arulkumar
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-21
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Scutellaria baicalensis Georgi has been used as traditional medicine for treating inflammatory diseases, hepatitis, tumors, and diarrhea in Asia.
Hence, we investigated the anti-inflammatory effect and determined the molecular mechanism of action of flavonoids isolated from Korean S.
baicalensis G.
in lipopolysaccharide- (LPS-) stimulated RAW 264.7 macrophages.
A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed to examine cytotoxicity of the flavonoids at various concentrations of 10, 40, 70, and 100 µg/mL.
No cytotoxicity was observed in RAW 264.7 cells at these concentrations.
Furthermore, the flavonoids decreased production of inflammatory mediators such as inducible nitric oxide synthase, cyclooxygenase-2, interleukin-6, and tumor necrosis factor-alpha and inhibited phosphorylation of nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinases (MAPKs) in LPS-induced RAW 264.7 cells.
Moreover, to identify the differentially expressed proteins in RAW 264.7 cells of the control, LPS-treated, and flavonoid-treated groups, two-dimensional gel electrophoresis and mass spectrometry were conducted.
The identified proteins were involved in the inflammatory response and included PRKA anchor protein and heat shock protein 70 kD.
These findings suggest that the flavonoids isolated from S.
baicalensis G.
might have anti-inflammatory effects that regulate the expression of inflammatory mediators by inhibiting the NF-κB signaling pathway via the MAPK signaling pathway in RAW 264.7 cells.
American Psychological Association (APA)
Hong, Gyeong-Eun& Kim, Jin-A.& Nagappan, Arulkumar& Yumnam, Silvia& Lee, Ho-Jeong& Kim, Eun-Hee…[et al.]. 2013. Flavonoids Identified from Korean Scutellaria baicalensis Georgi Inhibit Inflammatory Signaling by Suppressing Activation of NF-κB and MAPK in RAW 264.7 Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-507457
Modern Language Association (MLA)
Hong, Gyeong-Eun…[et al.]. Flavonoids Identified from Korean Scutellaria baicalensis Georgi Inhibit Inflammatory Signaling by Suppressing Activation of NF-κB and MAPK in RAW 264.7 Cells. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-507457
American Medical Association (AMA)
Hong, Gyeong-Eun& Kim, Jin-A.& Nagappan, Arulkumar& Yumnam, Silvia& Lee, Ho-Jeong& Kim, Eun-Hee…[et al.]. Flavonoids Identified from Korean Scutellaria baicalensis Georgi Inhibit Inflammatory Signaling by Suppressing Activation of NF-κB and MAPK in RAW 264.7 Cells. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-507457
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-507457