Pullulanase : Role in Starch Hydrolysis and Potential Industrial Applications
Joint Authors
Ling, Tau Chuan
Hii, Siew Ling
Tan, Joo Shun
Ariff, Arbakariya Bin
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-09-06
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
The use of pullulanase (EC 3.2.1.41) has recently been the subject of increased applications in starch-based industries especially those aimed for glucose production.
Pullulanase, an important debranching enzyme, has been widely utilised to hydrolyse the α-1,6 glucosidic linkages in starch, amylopectin, pullulan, and related oligosaccharides, which enables a complete and efficient conversion of the branched polysaccharides into small fermentable sugars during saccharification process.
The industrial manufacturing of glucose involves two successive enzymatic steps: liquefaction, carried out after gelatinisation by the action of α-amylase; saccharification, which results in further transformation of maltodextrins into glucose.
During saccharification process, pullulanase has been used to increase the final glucose concentration with reduced amount of glucoamylase.
Therefore, the reversion reaction that involves resynthesis of saccharides from glucose molecules is prevented.
To date, five groups of pullulanase enzymes have been reported, that is, (i) pullulanase type I, (ii) amylopullulanase, (iii) neopullulanase, (iv) isopullulanase, and (v) pullulan hydrolase type III.
The current paper extensively reviews each category of pullulanase, properties of pullulanase, merits of applying pullulanase during starch bioprocessing, current genetic engineering works related to pullulanase genes, and possible industrial applications of pullulanase.
American Psychological Association (APA)
Hii, Siew Ling& Tan, Joo Shun& Ling, Tau Chuan& Ariff, Arbakariya Bin. 2012. Pullulanase : Role in Starch Hydrolysis and Potential Industrial Applications. Enzyme Research،Vol. 2012, no. 2012, pp.1-14.
https://search.emarefa.net/detail/BIM-508305
Modern Language Association (MLA)
Hii, Siew Ling…[et al.]. Pullulanase : Role in Starch Hydrolysis and Potential Industrial Applications. Enzyme Research No. 2012 (2012), pp.1-14.
https://search.emarefa.net/detail/BIM-508305
American Medical Association (AMA)
Hii, Siew Ling& Tan, Joo Shun& Ling, Tau Chuan& Ariff, Arbakariya Bin. Pullulanase : Role in Starch Hydrolysis and Potential Industrial Applications. Enzyme Research. 2012. Vol. 2012, no. 2012, pp.1-14.
https://search.emarefa.net/detail/BIM-508305
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-508305