Development and Characterization of Polyphenon 60 and Caffeine Microemulsion for Enhanced Antibacterial Activity
Joint Authors
Dang, Shweta
Bansal, Rakhi
Gupta, Sonal
Ali, Javed
Gabrani, Reema
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-22
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Green tea catechins and caffeine have exhibited antibacterial activity; however, their use is limited by lack of stability and effective delivery systems.
Polyphenon 60 (P60) and caffeine were encapsulated in a single microemulsion (ME) formulation with an objective to lower the minimum inhibitory concentrations (MICs) of the individual agents against selected pathogens (S.
epidermidis and E.
coli).
Combination of two natural compounds would advocate two different mechanisms on the bacterial growth thereby providing for better antibacterial activity.
Thermodynamically stable ME was developed and characterized with an average particle size of 17.58 nm, further confirmed by TEM analysis.
Antibacterial studies included chequerboard microdilution assay to determine the MIC and fractional inhibitory concentration (FIC) of both the natural compounds individually and in combination.
MIC and FIC results indicated that the combination of the above two natural compounds was proficient in lowering the MICs of individual agents.
Results of DPPH assay indicated that ME system preserved the long term antioxidative potential of P60 and caffeine.
The cytotoxicity of the optimized formulation on Vero cell line by MTT assay was found to be nontoxic to mammalian cells.
American Psychological Association (APA)
Gupta, Sonal& Bansal, Rakhi& Ali, Javed& Gabrani, Reema& Dang, Shweta. 2014. Development and Characterization of Polyphenon 60 and Caffeine Microemulsion for Enhanced Antibacterial Activity. BioMed Research International،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-509219
Modern Language Association (MLA)
Gupta, Sonal…[et al.]. Development and Characterization of Polyphenon 60 and Caffeine Microemulsion for Enhanced Antibacterial Activity. BioMed Research International No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-509219
American Medical Association (AMA)
Gupta, Sonal& Bansal, Rakhi& Ali, Javed& Gabrani, Reema& Dang, Shweta. Development and Characterization of Polyphenon 60 and Caffeine Microemulsion for Enhanced Antibacterial Activity. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-509219
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-509219