Rapid Diagnosis of Pulmonary and Extrapulmonary Tuberculosis in HIV-Infected Patients. Comparison of LED Fluorescent Microscopy and the GeneXpert MTBRIF Assay in a District Hospital in India
Joint Authors
Naik, Praveen Kumar
Alvarez-Uria, Gerardo
Reddy, Raghuprakash
Midde, Manoranjan
Reddy, Srinivasulu
Azcona, Jose M.
Source
Tuberculosis Research and Treatment
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-08-26
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
HIV-related tuberculosis is difficult to diagnose and is associated with high morbidity and mortality.
Recently, the World Health Organization has endorsed the GeneXpert MTB/RIF (Xpert) assay for the diagnosis of pulmonary tuberculosis in HIV-infected patients from developing countries, but information about the use of Xpert for the diagnosis of extrapulmonary tuberculosis is scarce.
In this study, we compared the performance of light-emitting diode (LED) auramine fluorescent microscopy and the Xpert assay for the diagnosis of tuberculosis in HIV infected patients in a district hospital of India.
Although at higher cost, Xpert outperformed LED fluorescent microscopy in all type of specimens, especially in cerebrospinal fluid where the number of positive results was increased 11 times.
Pleural fluid, ascitic fluid, pus, and stool specimens also yielded positive results with the Xpert assay.
When collecting two additional early-morning sputum samples, the increase of the number of positive results with the Xpert assay was lower than previously reported for HIV infected patients.
Rifampicin resistance was observed in 2.2% of the cases.
The results of this study show that the Xpert assay can dramatically improve the rapid diagnosis of tuberculous meningitis and other types of extrapulmonary tuberculosis of HIV infected patients.
American Psychological Association (APA)
Alvarez-Uria, Gerardo& Azcona, Jose M.& Midde, Manoranjan& Naik, Praveen Kumar& Reddy, Srinivasulu& Reddy, Raghuprakash. 2012. Rapid Diagnosis of Pulmonary and Extrapulmonary Tuberculosis in HIV-Infected Patients. Comparison of LED Fluorescent Microscopy and the GeneXpert MTBRIF Assay in a District Hospital in India. Tuberculosis Research and Treatment،Vol. 2012, no. 2012, pp.1-4.
https://search.emarefa.net/detail/BIM-509327
Modern Language Association (MLA)
Alvarez-Uria, Gerardo…[et al.]. Rapid Diagnosis of Pulmonary and Extrapulmonary Tuberculosis in HIV-Infected Patients. Comparison of LED Fluorescent Microscopy and the GeneXpert MTBRIF Assay in a District Hospital in India. Tuberculosis Research and Treatment No. 2012 (2012), pp.1-4.
https://search.emarefa.net/detail/BIM-509327
American Medical Association (AMA)
Alvarez-Uria, Gerardo& Azcona, Jose M.& Midde, Manoranjan& Naik, Praveen Kumar& Reddy, Srinivasulu& Reddy, Raghuprakash. Rapid Diagnosis of Pulmonary and Extrapulmonary Tuberculosis in HIV-Infected Patients. Comparison of LED Fluorescent Microscopy and the GeneXpert MTBRIF Assay in a District Hospital in India. Tuberculosis Research and Treatment. 2012. Vol. 2012, no. 2012, pp.1-4.
https://search.emarefa.net/detail/BIM-509327
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-509327