Immunorecognition of the 14F7 Mab Raised against N-Glycolyl GM3 Ganglioside in Some Normal and Malignant Tissues from Genitourinary System
Joint Authors
Calzado, Enrique Rengifo
Cedeño, Mercedes
Carr, Adriana
Alvarez, Rosa Irene
Rengifo, Charles E.
Blanco, Rancés
Frómeta, Milagros
Escobar, Xiomara
Blanco, Damián
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-10-05
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
N-glycolyl neuraminic acid has been considered as a tumour-associated antigen forming both glycolipid and glycoprotein, expressed in some human malignant cells.
In this work, we evaluate the 14F7 Mab (an IgG1 murine highly specific to N-glycolyl GM3 ganglioside) reactivity in a variety of genitourinary-system-derived tumors as well as in their normal counterparts.
Immunohistochemical assays with 14F7 followed by anti-mouse biotinylated antibody and ABC/HRP system using formalin-fixed and paraffin-embedded tissues were carried out.
In normal tissues, 14F7 was reactive only in renal tubules of kidney (2/6) and in the stromal component and blood vessels of ovary (3/5).
Tumors of kidney (12/38), urinary bladder (8/9), breast (41/42), ovary (21/34), testis (4/5), prostate (17/20), and uterus (5/14) as well as prostatic nodular hyperplasia (5/8) were stained with 14F7.
N-glycolyl GM3 recognized by 14F7 could be considered as one attractive target for both active and passive immunotherapy of genitourinary malignancies expressing this molecule.
American Psychological Association (APA)
Blanco, Rancés& Cedeño, Mercedes& Escobar, Xiomara& Blanco, Damián& Rengifo, Charles E.& Frómeta, Milagros…[et al.]. 2011. Immunorecognition of the 14F7 Mab Raised against N-Glycolyl GM3 Ganglioside in Some Normal and Malignant Tissues from Genitourinary System. ISRN Pathology،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-511088
Modern Language Association (MLA)
Blanco, Rancés…[et al.]. Immunorecognition of the 14F7 Mab Raised against N-Glycolyl GM3 Ganglioside in Some Normal and Malignant Tissues from Genitourinary System. ISRN Pathology No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-511088
American Medical Association (AMA)
Blanco, Rancés& Cedeño, Mercedes& Escobar, Xiomara& Blanco, Damián& Rengifo, Charles E.& Frómeta, Milagros…[et al.]. Immunorecognition of the 14F7 Mab Raised against N-Glycolyl GM3 Ganglioside in Some Normal and Malignant Tissues from Genitourinary System. ISRN Pathology. 2011. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-511088
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-511088