Protective Effects of Shen-Yuan-Dan, a Traditional Chinese Medicine, against Myocardial IschemiaReperfusion Injury In Vivo and In Vitro
Joint Authors
Chu, Fuyong
Wu, Bao
Yang, Hongzhi
Tong, Tong
Li, Aiyong
Liu, Weihong
Shang, Juju
Liu, Hong-Xu
Xie, Xinran
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-23
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Objectives.
The study was to investigate the effects and mechanisms of Shen-Yuan-Dan (SYD) pharmacological postconditioning on myocardial ischemia/reperfusion (I/R) injury.
Methods.
In the in vivo experiment, myocardial injury markers and histopathology staining were examined.
In the in vitro experiment, cell viability and cell apoptosis were, respectively, detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays and Hoechst 33342 fluorochrome staining.
The protein expressions of Bcl-2 and Bax were determined by immunocytochemistry assay.
Results.
Both low and high doses of SYD protected myocardium against I/R injury in rat model by reducing lactic dehydrogenase (LDH) and creatine kinase-MB (CK-MB) activity and malondialdehyde (MDA) content, increasing superoxide dismutase (SOD) activity and attenuating histopathology injury.
Meanwhile, in the in vitro experiment, SYD promoted cell viability and inhibited the cardiomyocyte apoptosis.
The level of Bcl-2 protein was restored to the normal level by SYD pharmacological postconditioning.
In contrast, the Bax protein level was markedly reduced by SYD pharmacological postconditioning.
These effects of SYD were inhibited by LY294002.
Conclusions.
The results of this study suggested that SYD pharmacological postconditioning has protective effects against myocardial I/R injury in both in vivo and in vitro models, which are related to activating the phosphatidylinositol 3-kinase/Akt (PI3K/Akt) pathway.
American Psychological Association (APA)
Liu, Hong-Xu& Shang, Juju& Chu, Fuyong& Li, Aiyong& Wu, Bao& Xie, Xinran…[et al.]. 2013. Protective Effects of Shen-Yuan-Dan, a Traditional Chinese Medicine, against Myocardial IschemiaReperfusion Injury In Vivo and In Vitro. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-511237
Modern Language Association (MLA)
Liu, Hong-Xu…[et al.]. Protective Effects of Shen-Yuan-Dan, a Traditional Chinese Medicine, against Myocardial IschemiaReperfusion Injury In Vivo and In Vitro. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-511237
American Medical Association (AMA)
Liu, Hong-Xu& Shang, Juju& Chu, Fuyong& Li, Aiyong& Wu, Bao& Xie, Xinran…[et al.]. Protective Effects of Shen-Yuan-Dan, a Traditional Chinese Medicine, against Myocardial IschemiaReperfusion Injury In Vivo and In Vitro. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-511237
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-511237