Study on the Imprinting Status of Insulin-Like Growth Factor II (IGF-II)‎ Gene in Villus during 6–10 Gestational Weeks

Abstract EN

Objective.

To compare the difference of imprinting status of insulin-like growth factor II (IGF-II) gene in villus between normal embryo development group and abnormal embryo development group and to investigate the relationship between karyotype and the imprinting status of IGF-II gene.

Methods.

A total of 85 pregnant women with singleton pregnancy were divided into two groups: one with abnormal embryo development (n=38) and the other with normal embryo development (n=47).

Apa I polymorphism of IGF-II gene in chorionic villus was assayed with reverse transcriptase polymerase chain reaction (RT-PCR) and restriction fragment length polymorphism (RFLP).

The relationship between chromosomal abnormal karyotype and IGF-II gene imprinting status was analyzed by primary cell culture and G-banding chromosomal karyotype analysis.

Results.

IGF-II imprinting loss rate was higher in the abnormal embryo development group than the normal embryo development group (44.7% versus 31.6%), but without significant difference (P>.05).

The percentage of abnormal chromosomes of chorionic villus in the abnormal embryo development group was 42.5%, in which IGF-II imprinting loss rate reached 64.7%.

No abnormal karyotypes were found in the normal embryo development group.

However, there was significant difference in IGF-II imprinting loss rate between two groups (P>.05).

Conclusion.

During weeks 6–10 of gestation, abnormal embryonic development is correlated with chromosomal abnormalities.

The imprinting status of IGF-II gene played important roles in embryonic development, and imprinting loss might be related to chromosomal abnormalities.