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Higenamine Combined with [6]-Gingerol Suppresses Doxorubicin-Triggered Oxidative Stress and Apoptosis in Cardiomyocytes via Upregulation of PI3KAkt Pathway
Joint Authors
Xu, Zhi-Wei
Wu, Wei-Kang
Liao, Xin-Xue
Chen, Yan-Ling
Guo, Hua-Lei
Zhuang, Xiao-Dong
Lu, Li-He
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-05
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Sini decoction is a well-known formula of traditional Chinese medicine, which has been used to treat cardiovascular disease for many years.
Previously, we demonstrated that Sini decoction prevented doxorubicin-induced heart failure in vivo.
However, its active components are still unclear.
Thus, we investigated the active components of Sini decoction and their cardioprotective mechanisms in the in vitro neonatal rat cardiomyocytes and H9c2 cell line models of doxorubicin-induced cytotoxicity.
Our results demonstrated that treatment with higenamine or [6]-gingerol increased viability of doxorubicine-injured cardiomyocytes.
Moreover, combined use of higenamine and [6]-gingerol exerted more profound protective effects than either drug as a single agent, with effects similar to those of dexrazoxane, a clinically approved cardiac protective agent.
In addition, we found that treatment with doxorubicin reduced SOD activity, increased ROS generation, enhanced MDA formation, induced release of LDH, and triggered the intrinsic mitochondria-dependent apoptotic pathway in cardiomyocytes, which was inhibited by cotreatment of higenamine and [6]-gingerol.
Most importantly, the cytoprotection of higenamine plus [6]-gingerol could be abrogated by LY294002, a PI3K inhibitor.
In conclusion, combination of higenamine and [6]-gingerol exerts cardioprotective effect against doxorubicin-induced cardiotoxicity through activating the PI3K/Akt signaling pathway.
Higenamine and [6]-gingerol may be the active components of Sini decoction.
American Psychological Association (APA)
Chen, Yan-Ling& Zhuang, Xiao-Dong& Xu, Zhi-Wei& Lu, Li-He& Guo, Hua-Lei& Wu, Wei-Kang…[et al.]. 2013. Higenamine Combined with [6]-Gingerol Suppresses Doxorubicin-Triggered Oxidative Stress and Apoptosis in Cardiomyocytes via Upregulation of PI3KAkt Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-14.
https://search.emarefa.net/detail/BIM-512355
Modern Language Association (MLA)
Chen, Yan-Ling…[et al.]. Higenamine Combined with [6]-Gingerol Suppresses Doxorubicin-Triggered Oxidative Stress and Apoptosis in Cardiomyocytes via Upregulation of PI3KAkt Pathway. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-14.
https://search.emarefa.net/detail/BIM-512355
American Medical Association (AMA)
Chen, Yan-Ling& Zhuang, Xiao-Dong& Xu, Zhi-Wei& Lu, Li-He& Guo, Hua-Lei& Wu, Wei-Kang…[et al.]. Higenamine Combined with [6]-Gingerol Suppresses Doxorubicin-Triggered Oxidative Stress and Apoptosis in Cardiomyocytes via Upregulation of PI3KAkt Pathway. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-14.
https://search.emarefa.net/detail/BIM-512355
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-512355