CB2 Receptor Activation Ameliorates the Proinflammatory Activity in Acute Lung Injury Induced by Paraquat
Joint Authors
Xiao, Li
Zhao, Hongyu
Zheng, Qiang
Liu, Zhenning
Wang, Yu
Zhao, Min
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-22
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Paraquat, a widely used herbicide, is well known to exhibit oxidative stress and lung injury.
In the present study, we investigated the possible underlying mechanisms of cannabinoid receptor-2 (CB2) activation to ameliorate the proinflammatory activity induced by PQ in rats.
JWH133, a CB2 agonist, was administered by intraperitoneal injection 1 h prior to PQ exposure.
After PQ exposure for 4, 8, 24, and 72 h, the bronchoalveolar lavage fluid was collected to determine levels of TNF-α and IL-1β, and the arterial blood samples were collected for detection of PaO2 level.
At 72 h after PQ exposure, lung tissues were collected to determine the lung wet-to-dry weight ratios, myeloperoxidase activity, lung histopathology, the protein expression level of CB2, MAPKs (ERK1/2, p38MAPK, and JNK1/2), and NF-κBp65.
After rats were pretreated with JWH133, PQ-induced lung edema and lung histopathological changes were significantly attenuated.
PQ-induced TNF-α and IL-1β secretion in BALF, increases of PaO2 in arterial blood, and MPO levels in the lung tissue were significantly reduced.
JWH133 could efficiently activate CB2, while inhibiting MAPKs and NF-κB activation.
The results suggested that activating CB2 receptor exerted protective activity against PQ-induced ALI, and it potentially contributed to the suppression of the activation of MAPKs and NF-κB pathways.
American Psychological Association (APA)
Liu, Zhenning& Wang, Yu& Zhao, Hongyu& Zheng, Qiang& Xiao, Li& Zhao, Min. 2014. CB2 Receptor Activation Ameliorates the Proinflammatory Activity in Acute Lung Injury Induced by Paraquat. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-512482
Modern Language Association (MLA)
Liu, Zhenning…[et al.]. CB2 Receptor Activation Ameliorates the Proinflammatory Activity in Acute Lung Injury Induced by Paraquat. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-512482
American Medical Association (AMA)
Liu, Zhenning& Wang, Yu& Zhao, Hongyu& Zheng, Qiang& Xiao, Li& Zhao, Min. CB2 Receptor Activation Ameliorates the Proinflammatory Activity in Acute Lung Injury Induced by Paraquat. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-512482
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-512482