Dissection of Mechanisms of a Chinese Medicinal Formula : Danhong Injection Therapy for Myocardial IschemiaReperfusion Injury In Vivo and In Vitro
Joint Authors
Duan, Jia-Lin
Quan, Wei
Guan, Yue
Guo, Chao
Yin, Ying
Wang, Yan-Hua
Wei, Guo
Zhu, Yan-rong
Wen, Ai-Dong
Weng, Yan
Zhou, Dan
Xi, Miao-Miao
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-03
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Traditional Chinese medicine uses a systemic treatment approach, targeting multiple etiological factors simultaneously.
Danhong injection (DHI), a very popular Chinese medicine injection, is reported to be effective for many cardiovascular conditions.
The primary active ingredients of DHI, and their systemic and interrelated mechanism have not been evaluated in an established myocardial ischemia/reperfusion (MI/R) model.
We identified the main active constituents in DHI, including hydroxysafflor yellow A (A), salvianolic acid B (B), and danshensu (C), by HPLC fingerprint analysis and assessed their effect on MI/R rats and cardiomyocytes.
These 3 compounds and DHI all decreased the levels of IL-1, TNF-α, and MDA, increased those of IL-10 and SOD activity in vivo and in vitro, and had antiapoptotic effects, as shown by flow cytometric analysis and TUNEL assay.
Moreover, these compounds increased phosphorylation of Akt and ERK1/2 in cardiomyocytes.
Interestingly, we found compound A exerted a more prominent anti-inflammatory effect than B and C, by decreasing NF-κB levels; compound B had more powerful antioxidative capacity than A and C, by increasing Nrf2 expression; compound C had stronger antiapoptotic ability than A and B, by lowering caspase-3 activity.
Our results elucidate the mechanisms by which DHI protects against MI/R induced injury.
American Psychological Association (APA)
Guan, Yue& Yin, Ying& Zhu, Yan-rong& Guo, Chao& Wei, Guo& Duan, Jia-Lin…[et al.]. 2013. Dissection of Mechanisms of a Chinese Medicinal Formula : Danhong Injection Therapy for Myocardial IschemiaReperfusion Injury In Vivo and In Vitro. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-512536
Modern Language Association (MLA)
Guan, Yue…[et al.]. Dissection of Mechanisms of a Chinese Medicinal Formula : Danhong Injection Therapy for Myocardial IschemiaReperfusion Injury In Vivo and In Vitro. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-512536
American Medical Association (AMA)
Guan, Yue& Yin, Ying& Zhu, Yan-rong& Guo, Chao& Wei, Guo& Duan, Jia-Lin…[et al.]. Dissection of Mechanisms of a Chinese Medicinal Formula : Danhong Injection Therapy for Myocardial IschemiaReperfusion Injury In Vivo and In Vitro. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-512536
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-512536