Specific Conditions for Resveratrol Neuroprotection against Ethanol-Induced Toxicity
Joint Authors
Barret, Luc
Lamarche, Frédéric
Nuiry, Laurence
Allibe, Nathalie
Gonthier, Brigitte
Cottet-Rousselle, Cécile
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-06-17
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Pharmacy, Health & Medical Sciences
Medicine
Abstract EN
Aims.
3,5,4′-Trihydroxy-trans-stilbene, a natural polyphenolic compound present in wine and grapes and better known as resveratrol, has free radical scavenging properties and is a potent protector against oxidative stress induced by alcohol metabolism.
Today, the mechanism by which ethanol exerts its toxicity is still not well understood, but it is generally considered that free radical generation plays an important role in the appearance of structural and functional alterations in cells.
The aim of this study was to evaluate the protective action of resveratrol against ethanol-induced brain cell injury.
Methods.
Primary cultures of rat astrocytes were exposed to ethanol, with or without a pretreatment with resveratrol.
We examined the dose-dependent effects of this resveratrol pretreatment on cytotoxicity and genotoxicity induced by ethanol.
Cytotoxicity was assessed using the MTT reduction test.
Genotoxicity was evidenced using single cell gel electrophoresis.
In addition, DNA staining with fluorescent dyes allowed visualization of nuclear damage using confocal microscopy.
Results.
Cell pretreatment with low concentrations of trans-resveratrol (0.1–10 μM) slowed down cell death and DNA damage induced by ethanol exposure, while higher concentrations (50–100 μM) enhanced these same effects.
No protection by cis-resveratrol was observed.
Conclusion.
Protection offered by trans-resveratrol against ethanol-induced neurotoxicity was only effective for low concentrations of this polyphenol.
American Psychological Association (APA)
Gonthier, Brigitte& Allibe, Nathalie& Cottet-Rousselle, Cécile& Lamarche, Frédéric& Nuiry, Laurence& Barret, Luc. 2012. Specific Conditions for Resveratrol Neuroprotection against Ethanol-Induced Toxicity. Journal of Toxicology،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-512629
Modern Language Association (MLA)
Gonthier, Brigitte…[et al.]. Specific Conditions for Resveratrol Neuroprotection against Ethanol-Induced Toxicity. Journal of Toxicology No. 2012 (2012), pp.1-11.
https://search.emarefa.net/detail/BIM-512629
American Medical Association (AMA)
Gonthier, Brigitte& Allibe, Nathalie& Cottet-Rousselle, Cécile& Lamarche, Frédéric& Nuiry, Laurence& Barret, Luc. Specific Conditions for Resveratrol Neuroprotection against Ethanol-Induced Toxicity. Journal of Toxicology. 2012. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-512629
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-512629