Targeting PML-RARα and Oncogenic Signaling Pathways by Chinese Herbal Mixture Tien-Hsien Liquid in Acute Promyelocytic Leukemia NB4 Cells
Joint Authors
Lai, Tung-Yuan
Chuang, Shuang-En
Liu, Chun-Yen
Yang, Chia-Ming
Chen, Suz-Wen
Lai, Gi-Ming
Yao, Chih-Jung
Yan, Kun-Huang
Yan, Jiann-Long
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-02-20
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Tien-Hsien Liquid (THL) is a Chinese herbal mixture that has been used worldwide as complementary treatment for cancer patients in the past decade.
Recently, THL has been shown to induce apoptosis in various types of solid tumor cells in vitro.
However, the underlying molecular mechanisms have not yet been well elucidated.
In this study, we explored the effects of THL on acute promyelocytic leukemia (APL) NB4 cells, which could be effectively treated by some traditional Chinese remedies containing arsenic trioxide.
The results showed THL could induce G2/M arrest and apoptosis in NB4 cells.
Accordingly, the decrease of cyclin A and B1 were observed in THL-treated cells.
The THL-induced apoptosis was accompanied with caspase-3 activation and decrease of PML-RARα fusion protein.
Moreover, DNA methyltransferase 1 and oncogenic signaling pathways such as Akt/mTOR, Stat3 and ERK were also down-regulated by THL.
By using ethyl acetate extraction and silica gel chromatography, an active fraction of THL named as EAS5 was isolated.
At about 0.5–1% of the dose of THL, EAS5 appeared to have most of THL-induced multiple molecular targeting effects in NB4 cells.
Based on the findings of these multi-targeting effects, THL might be regarding as a complementary and alternative therapeutic agent for refractory APL.
American Psychological Association (APA)
Yao, Chih-Jung& Yang, Chia-Ming& Chuang, Shuang-En& Yan, Jiann-Long& Liu, Chun-Yen& Chen, Suz-Wen…[et al.]. 2011. Targeting PML-RARα and Oncogenic Signaling Pathways by Chinese Herbal Mixture Tien-Hsien Liquid in Acute Promyelocytic Leukemia NB4 Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-513563
Modern Language Association (MLA)
Yao, Chih-Jung…[et al.]. Targeting PML-RARα and Oncogenic Signaling Pathways by Chinese Herbal Mixture Tien-Hsien Liquid in Acute Promyelocytic Leukemia NB4 Cells. Evidence-Based Complementary and Alternative Medicine No. 2011 (2011), pp.1-11.
https://search.emarefa.net/detail/BIM-513563
American Medical Association (AMA)
Yao, Chih-Jung& Yang, Chia-Ming& Chuang, Shuang-En& Yan, Jiann-Long& Liu, Chun-Yen& Chen, Suz-Wen…[et al.]. Targeting PML-RARα and Oncogenic Signaling Pathways by Chinese Herbal Mixture Tien-Hsien Liquid in Acute Promyelocytic Leukemia NB4 Cells. Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-513563
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-513563