Intracellular and Extracellular Redox Status and Free Radical Generation in Primary Immune Cells from Children with Autism
Joint Authors
Li, Jingyun
Seidel, Lisa
Melnyk, Stepan
Trusty, Timothy A.
Rose, Shannon
Pavliv, Oleksandra
James, S. Jill
Nick, Todd
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-11-24
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The modulation of the redox microenvironment is an important regulator of immune cell activation and proliferation.
To investigate immune cell redox status in autism we quantified the intracellular glutathione redox couple (GSH/GSSG) in resting peripheral blood mononuclear cells (PBMCs), activated monocytes and CD4 T cells and the extracellular cysteine/cystine redox couple in the plasma from 43 children with autism and 41 age-matched control children.
Resting PBMCs and activated monocytes from children with autism exhibited significantly higher oxidized glutathione (GSSG) and percent oxidized glutathione equivalents and decreased glutathione redox status (GSH/GSSG).
In activated CD4 T cells from children with autism, the percent oxidized glutathione equivalents were similarly increased, and GSH and GSH/GSSG were decreased.
In the plasma, both glutathione and cysteine redox ratios were decreased in autistic compared to control children.
Consistent with decreased intracellular and extracellular redox status, generation of free radicals was significantly elevated in lymphocytes from the autistic children.
These data indicate primary immune cells from autistic children have a more oxidized intracellular and extracellular microenvironment and a deficit in glutathione-mediated redox/antioxidant capacity compared to control children.
These results suggest that the loss of glutathione redox homeostasis and chronic oxidative stress may contribute to immune dysregulation in autism.
American Psychological Association (APA)
Rose, Shannon& Melnyk, Stepan& Trusty, Timothy A.& Pavliv, Oleksandra& Seidel, Lisa& Li, Jingyun…[et al.]. 2011. Intracellular and Extracellular Redox Status and Free Radical Generation in Primary Immune Cells from Children with Autism. Autism Research and Treatment،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-513815
Modern Language Association (MLA)
Rose, Shannon…[et al.]. Intracellular and Extracellular Redox Status and Free Radical Generation in Primary Immune Cells from Children with Autism. Autism Research and Treatment No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-513815
American Medical Association (AMA)
Rose, Shannon& Melnyk, Stepan& Trusty, Timothy A.& Pavliv, Oleksandra& Seidel, Lisa& Li, Jingyun…[et al.]. Intracellular and Extracellular Redox Status and Free Radical Generation in Primary Immune Cells from Children with Autism. Autism Research and Treatment. 2011. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-513815
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-513815