Floss Chrysanthemi Indici protects against hydroxyl-induced damages to DNA and MSCs via antioxidant mechanism
Joint Authors
Li, Xican
Hu, Qiuping
Jiang, Shuxia
Lin, Jian
Han, Lu
Hong, Yuling
Lu, Wenbiao
Gao, Yaoxiang
Chen, Dongfeng
Li, Fei
Source
Journal of Saudi Chemical Society
Issue
Vol. 19, Issue 4 (31 Jul. 2015), pp.454-460, 7 p.
Publisher
Publication Date
2015-07-31
Country of Publication
Saudi Arabia
No. of Pages
7
Main Subjects
Topics
Abstract EN
Flos Chrysanthemi Indici (FCI) is a Chinese herbal medicine used in China for over 2000 years.
In this study, its ethanol extract (EFCL) was found to protect against hydroxyl radical (OH) induced oxidative damages to mesenchymal stem cells (MSCs) and DNA.
To explore the mechanism, EFCL was further determined by chemical and antioxidant assays.
Folin–Ciocalteu colorimetric assay suggested that content of total phenolics was 93.85 ± 3.56 mg catechin/g and HPLC analysis indicated a content of 10.05 ± 1.44 mg/g as chlorogenic acid in EFCI.
Antioxidant assays revealed that EFCI could also scavenge O2 radical (IC50 58.74 ± 1.30 lg/mL), DPPH radical (IC50 46.46 ± 1.35 lg/mL) and ABTS+ radical (IC50 20.59 ± 0.52 lg/mL), bind Fe2+ (IC50 364.27± 19.57 lg/mL) and Cu2+ (IC50 734.77± 34.69 lg/mL), reduce Fe3+ (IC50 147.11 ±11.09 lg/mL) and Cu2+ (IC50 35.69 ±3.14 lg/mL).
On the basis of mechanistic analysis, we concluded that FCI can effectively protect against hydroxyl-induced damages to DNA and MSCs, therefore FCI shows promise as a possible therapeutic reagent for many diseases.
The effect may be mainly attributed to phenolics especially chlorogenic acid, which exerts the antioxidant action possibly through metal-chelating, and radical-scavenging which is via hydrogen atom transfer (HAT) and/or sequential electron proton transfer (SEPT) mechanisms.
American Psychological Association (APA)
Li, Xican& Hu, Qiuping& Jiang, Shuxia& Li, Fei& Lin, Jian& Han, Lu…[et al.]. 2015. Floss Chrysanthemi Indici protects against hydroxyl-induced damages to DNA and MSCs via antioxidant mechanism. Journal of Saudi Chemical Society،Vol. 19, no. 4, pp.454-460.
https://search.emarefa.net/detail/BIM-590433
Modern Language Association (MLA)
Li, Xican…[et al.]. Floss Chrysanthemi Indici protects against hydroxyl-induced damages to DNA and MSCs via antioxidant mechanism. Journal of Saudi Chemical Society Vol. 19, no. 4 (Jul. 2015), pp.454-460.
https://search.emarefa.net/detail/BIM-590433
American Medical Association (AMA)
Li, Xican& Hu, Qiuping& Jiang, Shuxia& Li, Fei& Lin, Jian& Han, Lu…[et al.]. Floss Chrysanthemi Indici protects against hydroxyl-induced damages to DNA and MSCs via antioxidant mechanism. Journal of Saudi Chemical Society. 2015. Vol. 19, no. 4, pp.454-460.
https://search.emarefa.net/detail/BIM-590433
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 459-460
Record ID
BIM-590433