Biochemical studies on the natamycin antibiotic produced by streptomyces lydicus : fermentation, extraction and biological activities
Joint Authors
al-Sayyid, A. S.
al-Dasuqi, M. A.
Hassan, M.
al-Jazzar, M.
Atta, Hussein M.
Source
Journal of Saudi Chemical Society
Issue
Vol. 19, Issue 4 (31 Jul. 2015), pp.360-371, 12 p.
Publisher
Publication Date
2015-07-31
Country of Publication
Saudi Arabia
No. of Pages
12
Main Subjects
Topics
Abstract EN
Natamycin ‘‘polyene’’ antibiotic was isolated from the fermentation broth of a Streptomyces strain No.
AZ-55.
According to the morphological, cultural, physiological and biochemical characteristics, and 16S rDNA sequence analysis, strain AZ-55 was identified as Streptomyces lydicus.
It is active in vitro against some microbial pathogens viz: Staphylococcus aureus, NCTC 7447; Bacillus subtilis, NCTC 1040; Bacillus pumilus, NCTC 8214 ; Micrococcus luteus, ATCC 9341; Escherichia coli, NCTC 10416; Klebsiella pneumonia, NCIMB 9111; Salmonella typhi and Pseudomonas aeruginosa, ATCC 10145; S.
cerevisiae, ATCC 9763; Candida albicans, IMRU 3669; Aspergillus flavus, IMI 111023; Aspergillus niger, IMI 31276; Aspergillus fumigatus, ATCC 16424; Fusarium oxysporum; Alternaria alternata and Rhizoctonia solani.
The active metabolite was extracted using chloroform (1:1, v/v) at pH 7.0.
The separation of the active ingredient of the antifungal agent and its purification were performed using both thin layer chromatography (TLC) and column chromatography (CC) techniques.
The physico-chemical characteristics of the purified antibiotic viz.
color, melting point, solubility, elemental analysis (C, H, N, O and S) and spectroscopic characteristics (UV absorbance and IR, mass & NMR spectra) have been investigated.
This analysis indicates a suggested empirical formula of C33H47NO13.
The chemical structural analysis with spectroscopic characteristics confirmed that the compound produced by S.
lydicus, AZ-55 is Natamycin ‘‘polyene’’ antibiotic.
American Psychological Association (APA)
Atta, Hussein M.& al-Sayyid, A. S.& al-Dasuqi, M. A.& Hassan, M.& al-Jazzar, M.. 2015. Biochemical studies on the natamycin antibiotic produced by streptomyces lydicus : fermentation, extraction and biological activities. Journal of Saudi Chemical Society،Vol. 19, no. 4, pp.360-371.
https://search.emarefa.net/detail/BIM-590485
Modern Language Association (MLA)
Atta, Hussein M.…[et al.]. Biochemical studies on the natamycin antibiotic produced by streptomyces lydicus : fermentation, extraction and biological activities. Journal of Saudi Chemical Society Vol. 19, no. 4 (Jul. 2015), pp.360-371.
https://search.emarefa.net/detail/BIM-590485
American Medical Association (AMA)
Atta, Hussein M.& al-Sayyid, A. S.& al-Dasuqi, M. A.& Hassan, M.& al-Jazzar, M.. Biochemical studies on the natamycin antibiotic produced by streptomyces lydicus : fermentation, extraction and biological activities. Journal of Saudi Chemical Society. 2015. Vol. 19, no. 4, pp.360-371.
https://search.emarefa.net/detail/BIM-590485
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p.370-371
Record ID
BIM-590485