Biochemical studies of beta-2-microglobulin and its receptors in some breast tumors using radio receptor assay technique
Other Title(s)
دراسات كيميائية حياتية للكلوبيولين الدقيق بيتا الثاني (β2M) و مستلماته في بعض أورام الثدي باستخدام تقنية المستلم الإشعاعي
Dissertant
al-Birmani, Hasanayn Kamil Mahdi
Thesis advisor
University
University of Baghdad
Faculty
College of Science
Department
Chemistry Department
University Country
Iraq
Degree
Ph.D.
Degree Date
2005
English Abstract
Radioimmuno assay was used to measure the levels of Beta-2-microglobulin (b2M) in the sera of three women groups, group I consisted of (28) post-menopausal women with malignant breast tumor, group II consisted of (22) pre-menopausal women with malignant breast tumor and group III consisted of (25) pre-menopausal benign breast tumors.
A fourth group of (20) healthy women was also included as control.
The results showed significant differences between each group of cancer patients and the control group (P < 0.001).
The binding of 125I-b2M to its receptors in the cytosolic fraction of breast tumor homogenates was studied by using the radioimmuno assay technique with our modification for tissue ( radioreceptor assay).
The receptors for b2M were isolated from malignant post-menopausal homogenate using gel filtration chromatography technique.
Two forms of b2M receptors were found (protein I and protein II), which have a molecular weight 52.8 and 29 KD respectively.
The isolation process caused an increase in the binding affinity whereas the purification fold was 6 for protein I and 4.5 for protein II.
Time course and kinetic studies on the binding of 125I-b2M to its crude and isolated receptors were carried out at different temperatures.
The binding reaction is a time and temperature dependent process and follows the second order rate law in all studied samples.
The thermodynamic parameters of the standard state (DHo, DSo and DGo) and the transition state (DH*, DS* and DG*) and activation energy (Ea) were determined for 125I-b2M binding to its receptors at different temperatures.
These parameters were used to characterize the binding reaction that was found to be endothermic and spontaneous in all studied groups.
The spectroscopic characterization of b2M and (125I-b2M/receptor) complex was studied using ultraviolet spectroscopy.
Factors affecting the absorption spectra of the two species such as medium pH and solvent polarity were investigated.
The pKa values of histidine and tyrosine residues and the percentages for the presence of these residues on the surface of the two species (b2M and 125I-b2M / receptor complex) were studied using spectroscopic pH titration.
Main Subjects
Topics
No. of Pages
127
Table of Contents
Table of contents.
Abstract.
Abstract in Arabic.
Chapter One : General Introduction and literature survey.
Chapter Two : Preliminary studies on 125I-β2M binding to its receptors in breast tumor homogenates.
Chapter Three : Isolation of β2M receptors by gel filtration chromatography.
Chapter Four : Kinetic and thermodynamic studies on the binding of 125I-β2M to its receptors.
Chapter Five : Spectroscopic studies on β2M and (125I-β2M / receptor) complex.
Conclusions and future work.
References.
American Psychological Association (APA)
al-Birmani, Hasanayn Kamil Mahdi. (2005). Biochemical studies of beta-2-microglobulin and its receptors in some breast tumors using radio receptor assay technique. (Doctoral dissertations Theses and Dissertations Master). University of Baghdad, Iraq
https://search.emarefa.net/detail/BIM-600470
Modern Language Association (MLA)
al-Birmani, Hasanayn Kamil Mahdi. Biochemical studies of beta-2-microglobulin and its receptors in some breast tumors using radio receptor assay technique. (Doctoral dissertations Theses and Dissertations Master). University of Baghdad. (2005).
https://search.emarefa.net/detail/BIM-600470
American Medical Association (AMA)
al-Birmani, Hasanayn Kamil Mahdi. (2005). Biochemical studies of beta-2-microglobulin and its receptors in some breast tumors using radio receptor assay technique. (Doctoral dissertations Theses and Dissertations Master). University of Baghdad, Iraq
https://search.emarefa.net/detail/BIM-600470
Language
English
Data Type
Arab Theses
Record ID
BIM-600470
