Comparative study between the effect of stem cells and Metacam on induced arthritis in dogs

Abstract EN

Objective of this project was to study the effect of stem cells on the induced arthritis in the dogs as compared with treatment Metacam®.

Incomplete fruend's adjuvant was used to induce arthritis.

Twenty five dogs aged 12 month were divided into five groups: 1st group (T1) was negative control, while 2nd group (T2) was induced without any treatment (positive control), 3rd group (T3) was induction arthritis and treated with 0.2 mg/kg B.W.

of Metacam® intravenously (I/V) injection, 4th group (T4) was induced and treated with 1ml/kg of B.W of suspension of culture media injected (I / V), and 5th group (T5) was treated with stem cells suspension in a dose of 2.5 x 106 cells / ml / Kg B.W.

(I/V) injection.

Typical clinical signs of arthritis appeared on all induction groups.

The results of Hb and RBCs showed clear reduction in all induction groups as compared with (T1), the WBCs, Erythrocyte sediment rates (ESR) and C- reactive protein (CRP) in (T2) showed clear elevation as compared with (T1), After 15 and 30 days of treatment the RBCs count and HB level showed significant decline in T2, T3 and T4 as compared with T5 and T1, the WBCs ,ESR and CRP significantly elevated in the T2 and T4 as compared with T1.

The T3 and T5 showed clear depletion in levels of WBCs, ESR and CRP as compared with T2 after 15 and 30 days of treatment, while the T5 revealed no significant difference as compared with T1 after 30 days of treatment.

The histopathological changes in T2 and T4 showed erosion and infiltration of inflammatory cells, T3 showed hypertrophy of synovial lining and mild inflammatory cells in synovium, T5 explained return the bone tissue to normal.

In this experiment concluded a successful use of stem cells in treatment of arthritis without side effect as comparison with Metacam