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Detection of TET2, KRAS and CBL variants by next generation sequencing and analysis of their correlation with JAK2 and FLT3 in childhood AML
Joint Authors
Akar, Nejat
Oner, Deniz Aslar
Bahce, Muhterem
Kurekci, Imin
Akin, Dilara Fatimah
Mumcuoglu, Mine
Ezer, Ustun
Source
The Egyptian Journal of Medical Human Genetics
Issue
Vol. 17, Issue 2 (30 Apr. 2016), pp.209-215, 7 p.
Publisher
Egyptian Society of Human Genetics
Publication Date
2016-04-30
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Background: Acute myeloid leukemia (AML) is a heterogeneous clonal disorder in terms of cytogenetic and molecular aberrations.
Ten-Eleven-Translocation 2 (TET2), Kirsten rat sarcoma viral oncogene homolog (KRAS), and Casitas B-cell lymphoma (CBL) have an important role pathogenesis of acute myeloid leukemia (AML) and their activated mutations confer proliferative and survival signals.
Aim: In this study, we aimed to find possible genetic markers for molecular analysis in childhood AML by screening hot-spot exons of TET2, KRAS, and CBL using Next Generation Sequencing (NGS) analysis.
In addition, association between found variants and mutations of Januse Kinase-2 (JAK2) and Fms-Related Tyrosine Kinase (FLT3) were analyzed which are important prognostic risk factors for AML.
Methods: Eight patients who were diagnosed with pediatric AML at Losante Pediatric Hematology– Oncology Hospital were included to the study.
Hot-spot exons of TET2, KRAS and CBL genes were screened using the NGS method.
Furthermore, FLT3-Internal Tandem Duplicate (FLT3-ITD) and JAK2-V617F were analyzed by Real Time Polymerase chain Reaction (Real Time-PCR).
American Psychological Association (APA)
Akin, Dilara Fatimah& Oner, Deniz Aslar& Mumcuoglu, Mine& Ezer, Ustun& Bahce, Muhterem& Kurekci, Imin…[et al.]. 2016. Detection of TET2, KRAS and CBL variants by next generation sequencing and analysis of their correlation with JAK2 and FLT3 in childhood AML. The Egyptian Journal of Medical Human Genetics،Vol. 17, no. 2, pp.209-215.
https://search.emarefa.net/detail/BIM-679318
Modern Language Association (MLA)
Akin, Dilara Fatimah…[et al.]. Detection of TET2, KRAS and CBL variants by next generation sequencing and analysis of their correlation with JAK2 and FLT3 in childhood AML. The Egyptian Journal of Medical Human Genetics Vol. 17, no. 2 (Apr. 2016), pp.209-215.
https://search.emarefa.net/detail/BIM-679318
American Medical Association (AMA)
Akin, Dilara Fatimah& Oner, Deniz Aslar& Mumcuoglu, Mine& Ezer, Ustun& Bahce, Muhterem& Kurekci, Imin…[et al.]. Detection of TET2, KRAS and CBL variants by next generation sequencing and analysis of their correlation with JAK2 and FLT3 in childhood AML. The Egyptian Journal of Medical Human Genetics. 2016. Vol. 17, no. 2, pp.209-215.
https://search.emarefa.net/detail/BIM-679318
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 215
Record ID
BIM-679318