Hepatic and renal toxicity of methamidophos in male domestic rabbit
Other Title(s)
أثر سمية التمارون علي الكبد و الكلية في ذكور الأرانب المحلية
Dissertant
al-Najjar, Muhammad Samir Shahdah
Thesis advisor
Comitee Members
al-Habibi, Mahmud Ismail
al-Nabris, Kamal Jad Allah
University
Islamic University
Faculty
Faculty of Science
Department
Department of Biological Science
University Country
Palestine (Gaza Strip)
Degree
Master
Degree Date
2015
English Abstract
Objective: The present study is aimed to investigate hepatic and renal toxicity of methamidophos in male domestic rabbit.
Materials and Methods: The oral LD50 of methamidophos in male domestic rabbit was calculated and found to be 20.5 mg kg-1 body weight.
The daily dose of 1/10 LD50 methamidophos (2.1 mg kg-1 body weight) was given orally to 36 animals under experiment for six weeks.
Thirty six control animals were given distilled water.
Blood samples were collected weekly and analyzed.
Results: The overall mortality rate was 11.1% in methamidophos-treated rabbits compared to 0.0% in controls.
Clinical signs included diarrhea, disorientation, drowsiness, weakness, depression and mild tremors.
The final body weight was significantly decreased in methamidophos-intoxicated rabbits.
Serum glucose was significantly increased in response to methamidophos administration recording a maximum percentage difference of 27.1% in the 4th week of the experiment.
The activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and gamma glutamyl transferase (γ- GT) were significantly higher in methamidophos-fed rabbits compared to controls, registering maximum percentage differences of 31.2, 44.7, 26.7 and 45.6% during the 6th, 4th, 4th and 3rd weeks of the experiment.
In contrast, serum cholinesterase (ChE) was significantly decreased recording a maximum percentage difference of 77.8% at the end of the experiment.
Serum bilirubin was gradually increased to record a maximum percentage difference of 19.3% in the 5th week.
Serum urea and creatinine concentrations were significantly elevated in response to methamidophos intake displaying maximum percentage differences of 45.8% and 31.7% during the 4th week of the experiment.
Serum total protein, albumin and globulin were significantly decreased upon methamidophos intoxication exhibiting percentage differences of 27.0, 25.9 and 24.3% at 5th week of the experiment.
Serum calcium was significantly decreased in methamidophos-treated rabbits with a maximum percentage difference of 17.4% at the end of the experiment, whereas phosphorus was significantly increased with a maximum percentage difference of 23.8% at the 4th week of the experiment.
Conclusions: Oral daily administration of 1/10 LD50 methamidophos caused significant decrease in the body weight, serum cholinesterase, total protein, albumin, globulin and calcium whereas serum glucose, ALT, AST, ALP, -GT, bilirubin, urea, creatinine and phosphorus were significantly increased.
Main Subjects
Topics
No. of Pages
59
Table of Contents
Table of contents.
Abstract.
Abstract in Arabic.
Chapter One : Introduction.
Chapter Two : Literature review.
Chapter Three : Materials and methods.
Chapter Four : Results.
Chapter Five : Discussion.
Chapter Six : Conclusion.
References.
American Psychological Association (APA)
al-Najjar, Muhammad Samir Shahdah. (2015). Hepatic and renal toxicity of methamidophos in male domestic rabbit. (Master's theses Theses and Dissertations Master). Islamic University, Palestine (Gaza Strip)
https://search.emarefa.net/detail/BIM-687997
Modern Language Association (MLA)
al-Najjar, Muhammad Samir Shahdah. Hepatic and renal toxicity of methamidophos in male domestic rabbit. (Master's theses Theses and Dissertations Master). Islamic University. (2015).
https://search.emarefa.net/detail/BIM-687997
American Medical Association (AMA)
al-Najjar, Muhammad Samir Shahdah. (2015). Hepatic and renal toxicity of methamidophos in male domestic rabbit. (Master's theses Theses and Dissertations Master). Islamic University, Palestine (Gaza Strip)
https://search.emarefa.net/detail/BIM-687997
Language
English
Data Type
Arab Theses
Record ID
BIM-687997