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Bioprocess development for kefiran production by Lactobacillus kefiranofaciens in semi industrial scale bioreactor
Joint Authors
Dailin, Daniel Joe
al-Sayyid, al-Sayyid Ahmad
Uthman, Nur Zalina
Malik, Roslinda
Phin, Hiew Siaw
Wadaan, Muhammad
al-Inshasi, Hisham Ali
Aziz, Ramlan
Source
Saudi Journal of Biological Sciences
Issue
Vol. 23, Issue 4 (31 Jul. 2016), pp.495-502, 8 p.
Publisher
Publication Date
2016-07-31
Country of Publication
Saudi Arabia
No. of Pages
8
Main Subjects
Abstract EN
Lactobacillus kefiranofaciens is non-pathogenic gram positive bacteria isolated from kefir grains and able to produce extracellular exopolysaccharides named kefiran.
This polysaccharide contains approximately equal amounts of glucose and galactose.
Kefiran has wide applications in pharmaceutical industries.
Therefore, an approach has been extensively studied to increase kefiran production for pharmaceutical application in industrial scale.
The present work aims to maximize kefiran production through the optimization of medium composition and production in semi industrial scale bioreactor.
The composition of the optimal medium for kefiran production contained sucrose, yeast extract and K2HPO4 at 20.0, 6.0, 0.25 g L1, respectively.
The optimized medium significantly increased both cell growth and kefiran production by about 170.56% and 58.02%, respectively, in comparison with the unoptimized medium.
Furthermore, the kinetics of cell growth and kefiran production in batch culture of L.
kefiranofaciens was investigated under un-controlled pH conditions in 16-L scale bioreactor.
The maximal cell mass in bioreactor culture reached 2.76 g L1 concomitant with kefiran production of 1.91 g L1.
American Psychological Association (APA)
Dailin, Daniel Joe& al-Sayyid, al-Sayyid Ahmad& Uthman, Nur Zalina& Malik, Roslinda& Phin, Hiew Siaw& Aziz, Ramlan…[et al.]. 2016. Bioprocess development for kefiran production by Lactobacillus kefiranofaciens in semi industrial scale bioreactor. Saudi Journal of Biological Sciences،Vol. 23, no. 4, pp.495-502.
https://search.emarefa.net/detail/BIM-689574
Modern Language Association (MLA)
Dailin, Daniel Joe…[et al.]. Bioprocess development for kefiran production by Lactobacillus kefiranofaciens in semi industrial scale bioreactor. Saudi Journal of Biological Sciences Vol. 23, no. 4 (Jul. 2016), pp.495-502.
https://search.emarefa.net/detail/BIM-689574
American Medical Association (AMA)
Dailin, Daniel Joe& al-Sayyid, al-Sayyid Ahmad& Uthman, Nur Zalina& Malik, Roslinda& Phin, Hiew Siaw& Aziz, Ramlan…[et al.]. Bioprocess development for kefiran production by Lactobacillus kefiranofaciens in semi industrial scale bioreactor. Saudi Journal of Biological Sciences. 2016. Vol. 23, no. 4, pp.495-502.
https://search.emarefa.net/detail/BIM-689574
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 501-502
Record ID
BIM-689574