Optimum conditions for fibrinolytic enzyme (Nattokinase) production by Bacillus sp. B24 using solid state fermentation
Other Title(s)
تحديد الظروف المثلى لإنتاج الإنزيم المحلل لليفين (الناتوكاينيز) باستعمال Bacillus sp. B24 بوساطة تخمرات الحالة الصلبة
Joint Authors
Hamud, Sumayyah Ali
Aziz, Ghazi Munim
Source
Issue
Vol. 57, Issue 2C (30 Jun. 2016), pp.1391-1401, 11 p.
Publisher
University of Baghdad College of Science
Publication Date
2016-06-30
Country of Publication
Iraq
No. of Pages
11
Main Subjects
Topics
Abstract EN
The present study was aimed to screen the ability of local isolates of Bacillus spp.
(56 isolates) for nattokinase production using solid state fermentation, then optimize the nutritional conditions for enzyme production.
The isolates were subjected to the primary and secondary screening process to select the Bacillus isolate which give the highest production of enzyme.
It was found that Bacillus sp.
B24 had the highest productivity of the enzyme (25.58U/mg protein).
The optimum conditions for nattokinase production were performed by the solid state fermentation and found that the wheat bran was the best medium at initial moisture ratio 1.0 : 1.0 (w/v) using distilled water as moisturizing solution with initial pH of 7.0 after inoculation with 5×107 cell/ml for 72 hrs, the enzyme specific activity was 38.65U/mg protein.
American Psychological Association (APA)
Hamud, Sumayyah Ali& Aziz, Ghazi Munim. 2016. Optimum conditions for fibrinolytic enzyme (Nattokinase) production by Bacillus sp. B24 using solid state fermentation. Iraqi Journal of Science،Vol. 57, no. 2C, pp.1391-1401.
https://search.emarefa.net/detail/BIM-699573
Modern Language Association (MLA)
Hamud, Sumayyah Ali& Aziz, Ghazi Munim. Optimum conditions for fibrinolytic enzyme (Nattokinase) production by Bacillus sp. B24 using solid state fermentation. Iraqi Journal of Science Vol. 57, no. 2C (2016), pp.1391-1401.
https://search.emarefa.net/detail/BIM-699573
American Medical Association (AMA)
Hamud, Sumayyah Ali& Aziz, Ghazi Munim. Optimum conditions for fibrinolytic enzyme (Nattokinase) production by Bacillus sp. B24 using solid state fermentation. Iraqi Journal of Science. 2016. Vol. 57, no. 2C, pp.1391-1401.
https://search.emarefa.net/detail/BIM-699573
Data Type
Journal Articles
Language
English
Notes
Text in English ; abstracts in English and Arabic.
Record ID
BIM-699573