Determination the activity of some physical and chemical factors on the polyphenol oxidase in hot pepper and cabbage (brassica oleracea var)
Joint Authors
Ismail, Haydar Haqqi
Abbas, Ayat Adnan
Abd al-Latif, Muhammad Umar
Source
Iraqi Journal of Biotechnology
Issue
Vol. 15, Issue 1 (30 Jun. 2016), pp.53-60, 8 p.
Publisher
University of Baghdad Genetic Engineering and Biotechnology for Postgraduate Studies
Publication Date
2016-06-30
Country of Publication
Iraq
No. of Pages
8
Main Subjects
Topics
Abstract EN
The activity of polyphenol oxidase (PPO) in hot pepper pericarp and cabbage was evaluated using spectrophotometric method.
The enzyme was extracted from the pepper pericarp with suitable volume of 0.1 M phosphate buffer solution pH (7.
0) .PPO activity was determined using catechol as a substrate.
The effects of the enzyme concentration, substrate concentration, pH and temperature were investigated.
The highest activity of ppo at (2.5 mg /ml) from hot pepper and the maximum activity of ppo from cabbage was at (1.5 mg /ml) .The highest activity of enzyme catechol concentration was at 100 mM for PPO from hot pepper and cabbage respectively.
The optimum pH was 6.0 and 7.0 for PPO from hot pepper and cabbage.
The optimum temperatures were 40°C, 30 °C for hot pepper and cabbage respectively.
These optimum conditions were used to determine the enzyme activity in hot pepper and cabbage.
American Psychological Association (APA)
Abbas, Ayat Adnan& Ismail, Haydar Haqqi& Abd al-Latif, Muhammad Umar. 2016. Determination the activity of some physical and chemical factors on the polyphenol oxidase in hot pepper and cabbage (brassica oleracea var). Iraqi Journal of Biotechnology،Vol. 15, no. 1, pp.53-60.
https://search.emarefa.net/detail/BIM-699654
Modern Language Association (MLA)
Abbas, Ayat Adnan…[et al.]. Determination the activity of some physical and chemical factors on the polyphenol oxidase in hot pepper and cabbage (brassica oleracea var). Iraqi Journal of Biotechnology Vol. 15, no. 1 (2016), pp.53-60.
https://search.emarefa.net/detail/BIM-699654
American Medical Association (AMA)
Abbas, Ayat Adnan& Ismail, Haydar Haqqi& Abd al-Latif, Muhammad Umar. Determination the activity of some physical and chemical factors on the polyphenol oxidase in hot pepper and cabbage (brassica oleracea var). Iraqi Journal of Biotechnology. 2016. Vol. 15, no. 1, pp.53-60.
https://search.emarefa.net/detail/BIM-699654
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 59-60
Record ID
BIM-699654