Antiepileptic and anticonvulsant effect of bongardia chrysogonum on pentylenetetrazol-induced kindling in mice

Other Title(s)

تقييم مستخلص درنات نبتة عرف الديك كمضاد للصرع في الفئران

Dissertant

al-Shahir, Fadwa Thair

Thesis advisor

Abu Hamdah, Sawsan
Abu Rumaylah, Amjad Naji

Comitee Members

Nusayr, Khawlah Qasim
al-Abd, Wadi Tawfiq

University

Isra University

Faculty

Faculty of Pharmacy

University Country

Jordan

Degree

Master

Degree Date

2016

English Abstract

Epilepsy is a diverse set of recurrent chronic neurological disorder which causes substantial mortality and morbidity.

Side effects of common drug regimens used in the treatment of epilepsy are high.

Furthermore, high percentage of seizure types are resistant to well-known drug therapies.

Therefore it is necessary to find new antiepileptic therapies with low side effects that can control various seizure types.

In traditional Jordanian folk medicine, Bongardia chrysogonum tubers are used for the treatment of epilepsy.

In this study kindling method using pentylenetetrazole induced seizures was used to investigate BC tubers ability to reduce the severity of seizures induced by PTZ in mice.

Mice were divided into nine groups, each group included 6 mice.

3 groups were treated by BC water extracts with doses of 600, 900 and 1200 mg/kg of body weight followed by a dose of PTZ, 3 groups were treated by ethanol extracts with doses of 600, 900 and 1200 mg/kg of body weight followed by a dose of PTZ, a group treated with valproic acid with a dose of 200 mg/kg of body weight, a group is given distilled water of a dose of 10 ml/kg of body weight followed by a dose of PTZ, a group were given distilled water only with a dose of 10 mg/kg of body weight.

Kindling was performed in 11 doses every other day.

In the first 10 doses, all groups except the negative control groups were given a dose of 40 mg/kg of PTZ after 30 to 60 minutes of treatment with water extract, ethanol extract and valproic acid.

In the 11thdose also called the challenge dose, all groups were given a dose of 75 mg/kg of PTZ 30 to 60 30 to 60 minutes of treatment with water extract, ethanol extract and valproic acid.

The behavior scale is made by monitoring the mice after giving them the PTZ dose.

Results showed that BC tubers have antiepileptic effect with high protection values in the challenge day in dose dependent manner.

Water extract groups showed higher protections than ethanol extract groups.

Statistical analysis showed significant results between BC water extract groups and PTZ group and between BC ethanol extract groups and PTZ group (p< 0.05).

Antiepileptic effect of BC may be reduced to the presence of previously found active constituents in the tubers working by GABA agonist or NMDA antagonist mechanisms.

Main Subjects

Pharmacy, Health & Medical Sciences

No. of Pages

46

Table of Contents

• APA
• MLA
• AMA

Language

English

Data Type

Arab Theses

Record ID

BIM-707206