The protective effect of selinium in methotrexate induced nephrotoxicity in Rabbits

Abstract EN

Methotrexate (MTX), a cytotoxic chemotherapeutic agent, is used in the treatment of acute lymphoblastic leukaemia, lymphoma, osteosarcoma, breast cancer, head and neck cancers and also in the therapy of non oncologic disorders such as rheumatic diseases and psoriasis.

The aim of the present study was to examine the protective effect of selenium against the MTX-induced nephrotoxicity.

Materials and Methods: 21 rabbits were used and randomized in to three groups (7 rabbits each) ,all groups were maintained on standard chow diet throughout the experiment (8 weeks).

Group 1 was treated with normal saline water (control), Group 2 with MTX (nephrotoxic) and Group 3 with MTX plus selinium.

Induction of nephrotoxicity was carried out by administration of MTX to the rabbits in a dose of 0.25 mg/kg /day i.m for 8 weeks.

Blood urea and serum creatinin were measured to asses renal function.

Serum MDA levels and SOD activity also performed.

Results: Eight weeks of MTX treatment produced significant changes in renal function markers.

Further it was found that serum MDA levels and SOD activity were significantly increased, while serum GSH levels were significantly decreased.

Adding selinium to MTX was found to produced significant improvement in renal function .

Conclusion: selinium restored the altered renal function and possessed protection to the kidney against MTX induced nephrotoxicity