Isolation and identification of staphylococcus aureus, listeria monocytogenes, E. coli O157 :H7 and salmonella specious from raw beef and lamb meat in Baghdad by PCR
Other Title(s)
عزل و تشخيص جراثيم المكورات العنقودية الذهبية، اللستيريا، الاشريشيا القولونية و السالمونيلا من لحوم الأبقار و الأغنام النيئة في مدينة بغداد بواسطة تفاعل سلسلة البلمرة المتعدد
Author
Source
Issue
Vol. 57, Issue 3B (30 Sep. 2016), pp.1891-1897, 7 p.
Publisher
University of Baghdad College of Science
Publication Date
2016-09-30
Country of Publication
Iraq
No. of Pages
7
Main Subjects
Topics
- Meat
- Foodborne diseases
- Antibiotics
- Baghdad
- Lamb meat
- Pathogenic bacteria
- Polymerase chain reaction
- Staphylococcus
- Diagnosis
- Cows
- Molecular aspects
- Escherichia coli
- Listeria monocytogenes
Abstract EN
The study designed to determine the distribution of a major important food pathogens including Staphylococcus aureus , Listeria monocytogenes, E.
coli O157:H7 and Salmonella spp from raw beef and lamb meat by using multiplex pcr .
A total of 90 raw beef and lamb meat samples were collected from different butcher's shops in Al-Karkh side of Baghdad city and analyzed for the presence of these types of bacteria and their susceptibilities to some antibiotics was investigated ,the results showed that the prevalence of S.
aureus (5.6%), L.
monocytogenes (3%), E.
coli O157:H7 (7.8 %) and Salmonella spp (5.6%) from the total samples .The result of the susceptibility test showed that S.
aureus isolates were susceptible to Amikacin (80%) ,while L.
monocytogenes isolates were susceptible to the most used antibiotics as following Amikacin, Erythromycin, Oxytetracycline, Nalidixic acid ,Cephalothin, Gentamycin, Ampicillin and Streptomycin (100%).
E.
coli O157:H7 isolates were susceptible to Nalidixic acid and Gentamycin (100%) and Salmonella spp isolates were susceptible to Nalidixic acid ,Cephalothin and Gentamycin (80%) .
American Psychological Association (APA)
Khalil, Zaynah Qasim. 2016. Isolation and identification of staphylococcus aureus, listeria monocytogenes, E. coli O157 :H7 and salmonella specious from raw beef and lamb meat in Baghdad by PCR. Iraqi Journal of Science،Vol. 57, no. 3B, pp.1891-1897.
https://search.emarefa.net/detail/BIM-709481
Modern Language Association (MLA)
Khalil, Zaynah Qasim. Isolation and identification of staphylococcus aureus, listeria monocytogenes, E. coli O157 :H7 and salmonella specious from raw beef and lamb meat in Baghdad by PCR. Iraqi Journal of Science Vol. 57, no. 3B (2016), pp.1891-1897.
https://search.emarefa.net/detail/BIM-709481
American Medical Association (AMA)
Khalil, Zaynah Qasim. Isolation and identification of staphylococcus aureus, listeria monocytogenes, E. coli O157 :H7 and salmonella specious from raw beef and lamb meat in Baghdad by PCR. Iraqi Journal of Science. 2016. Vol. 57, no. 3B, pp.1891-1897.
https://search.emarefa.net/detail/BIM-709481
Data Type
Journal Articles
Language
English
Notes
Text in English ; abstracts in English and Arabic.
Record ID
BIM-709481