Diagnostic value of anti-peptidylarginine deiminase type 4 (PADI-4)‎ and anti-cyclic citrullinated peptide antibodies (ACCP)‎ among Iraqi patients with rheumatoid arthritis

Other Title(s)

القيمة التشخيصية لمضادات الببتيدايل آرجنين -4 و لمضادات الببتيدية الحلقية الحاوية على السترولين بين المرضى العراقيين المصابين بالتهاب المفاصل الرثوي

Dissertant

Badran, Hisham Abd al-Jalil

Thesis advisor

Hassuni, Hasan J.

Comitee Members

Hamdan, Thamir Ahmad
Mahmud, Khalid Hasan
al-Hamdani, Adnan Hamad Ubayd
al-Abbasi, Ahmad M.

University

University of Basrah

Faculty

Veterinary College

Department

Department of Microbiology

University Country

Iraq

Degree

Master

Degree Date

2014

English Abstract

Objective.

To examine the value of anti- peptidylarginine deiminase (anti-PADI-4) antibody and anti-cyclic citrullinated peptide (anti-CCP) antibody among Iraqi patients with RA and to determine whether the activity and severity in RA patients are associated with anti-PADI-4 and anti-CCP antibodies positivity .

Method.

In a case control study , we determined the seropositivity of these two serological markers anti-PADI-4 and anti-CCP antibodies in100 RA patients and 100 healthy control subjects .

Activity of disease were measured by disease activity score in 28 joints (DAS 28) and severity was assessed using Scott modification of the Larsen method.

Result.

Antibodies to PADI-4 were detected in 32 of 100 (32%)among RA patients and present 1 of 100 ( 1%) in controls (p < 0.001).

Anti-CCP was present in 74/100 (74%) of the RA patients and present in 2/100 (2%) in controls (p < 0.001) , sensitivity was highest for anti-CCP antibody (74%) followed by anti-PADI-4 antibody (32%).Specificity was highest for anti-PADI-4 antibody (99%) followed by anti-CCP antibody(98%).

A significant correlation with disease activity was observed in both markers , RA patients on remission had negative PADI-4 test (0/12) but patients with high disease activity showed higher percentage (86.4%) of PADI-4 seropositivity in comparison to anti-CCP where patients on remission had (8.3%) seropositivity and RA patients with high disease activity had (91%) anti-CCP seropositivity .

However , seropostivity to PADI-4 was significantly correlated with RA severity ( p < 0.001) as evaluated by scott method (59.3%) in grade 5 .

Similarly , there were a significant correlation between anti-CCP seropositivity and radiological finding but less than that associated with seropositivity to PADI-4 antibody (p < 0.05).

Conclusion.

With their excellent specificity , both anti-PADI-4 and anti-CCP antibodies can be useful in establishing the diagnosis of RA, and can be regarded as good predictors of disease activity and severity .

Main Subjects

Biology

No. of Pages

151

Table of Contents

• APA
• MLA
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Language

English

Data Type

Arab Theses

Record ID

BIM-744766