Micro RNA-146a expression as a potential biomarker for rheumatoid arthritis in Egypt
Joint Authors
Hamdi, Maha Salah Al-Din
Murshidi, Nashwh Ali
al-Sayyid, Hibah Muhammad Abd al-Qadir
Khatir, Wala Shawqi
Ibrahim, Ayman Asaad
Source
The Egyptian Journal of Medical Human Genetics
Issue
Vol. 18, Issue 2 (30 Apr. 2017), pp.173-179, 7 p.
Publisher
Egyptian Society of Human Genetics
Publication Date
2017-04-30
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
MicroRNAs (miRNAs) are small non-coding RNAs, whose role in regulating diverse immune functions, suggests they might play a role as biomarkers for immune mediated disorders.
Studies showed that miRNA-146a (miR-146a) expression is increased by proinflammatory cytokines and is an important modulator of differentiation and function of cells of innate and adaptive immunity.
Aim of the work: The current study aimed to evaluate the expression of miR-146a as a potential biomarker for diagnosis of rheumatoid arthritis (RA) and to explore its association with disease activity.
Subjects and methods: The study enrolled 50 Egyptian subjects divided into a patient group, which comprised 25 RA patients, and a control group which comprised 25 healthy individuals.
The disease activity for the patients’ group was determined by simplified disease activity index.
Relative quantification of miR-146a expression in whole blood was determined using reverse transcriptase quantitative real time polymerase chain reaction.
Results: There were highly significant statistical differences between patients and healthy controls as regards miR-146a relative expression, erythrocyte sedimentation rate (ESR) and anticyclic citrullinated peptide (anti-CCP) (p< 0.001).
Highly significant statistical differences (p< 0.001) were also found between different patients’ subgroups as regards miR-146a relative expression and ESR.
miR-146a levels correlated positively with those of ESR, C-reactive protein and anti-CCP (p< 0.001).
miR-146a illustrated best performance in diagnosing RA, showing the highest sensitivity and specificity (96% and 100%, respectively) (AUC: 0.992 at a cut off value of P2.16) compared to anti-CCP (sensitivity: 68%, specificity: 100% and AUC: 0.87 at a cut off value of P22 U/ml) and RF (sensitivity: 56%, specificity: 80% and AUC: 0.992 at a cut off value of P13 U/ml).
American Psychological Association (APA)
al-Sayyid, Hibah Muhammad Abd al-Qadir& Khatir, Wala Shawqi& Ibrahim, Ayman Asaad& Hamdi, Maha Salah Al-Din& Murshidi, Nashwh Ali. 2017. Micro RNA-146a expression as a potential biomarker for rheumatoid arthritis in Egypt. The Egyptian Journal of Medical Human Genetics،Vol. 18, no. 2, pp.173-179.
https://search.emarefa.net/detail/BIM-760949
Modern Language Association (MLA)
al-Sayyid, Hibah Muhammad Abd al-Qadir…[et al.]. Micro RNA-146a expression as a potential biomarker for rheumatoid arthritis in Egypt. The Egyptian Journal of Medical Human Genetics Vol. 18, no. 2 (Apr. 2017), pp.173-179.
https://search.emarefa.net/detail/BIM-760949
American Medical Association (AMA)
al-Sayyid, Hibah Muhammad Abd al-Qadir& Khatir, Wala Shawqi& Ibrahim, Ayman Asaad& Hamdi, Maha Salah Al-Din& Murshidi, Nashwh Ali. Micro RNA-146a expression as a potential biomarker for rheumatoid arthritis in Egypt. The Egyptian Journal of Medical Human Genetics. 2017. Vol. 18, no. 2, pp.173-179.
https://search.emarefa.net/detail/BIM-760949
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 178
Record ID
BIM-760949