Characterization of crude lipoxygenase activity from natural and callus extracts of carrot

Abstract EN

The present work was designed to characterize crude carrot lipoxygenase activity from natural and callus extract specially induced with help of plant growth regulators.

Characterization of carrot lipoxygenase offers the potential of rising scientific facts that can support the determination of optimum processing at which the detrimental effects of this enzyme are minimized.

Lipoxygenase activity was assayed by using spectrophotometric method.

Optimal activity of crude lipoxygenase was found at pH 6 and 7 for natural carrot and callus extracts respectively.

The kinetic parameters determined under the best conditions were a Michaelis constant (KM) of 0.004 M and 0.001 M, while the values obtained for the maximum rate (Vmax) were 1250 units and 1000 units for the lipoxygenase from natural carrot and callus extracts respectively.

Crude lipoxygenase activity that extract from natural and callu