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Leptin adversely affects the transition of histone-to-protamine during spermatogenesis in sprague-dawley rats
Other Title(s)
هرمون الليبتين على معدل تحول الهيستون إلى بروتامين في الحيوانات المنوية لدى الفئران البيضاء البالغة
Joint Authors
al-Mabhuh, Fayiz A. M.
Singh, Harbindar J.
Source
IUG Journal of Natural Studies
Issue
Vol. 25, Issue 2 (31 Dec. 2017), pp.13-20, 8 p.
Publisher
The Islamic University-Gaza Deanship of Research and Graduate Affairs
Publication Date
2017-12-31
Country of Publication
Palestine (Gaza Strip)
No. of Pages
8
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
The aim of this study was to examine the effect of leptin on the histone to protamine ratio in adult rat sperm.
Twelve-week old Sprague-Dawley rats were randomized into control and leptin-treated groups with 6 rats per group.
Leptin-treated group received intra-peritoneal injections (i.p.) of leptin daily for 42 days (60 μg/kg body weight).
Control rats received 0.1 ml of 0.9 % saline i.p.
for 42 days.
Upon completion of the treatment sperm count, morphology and histone-to-protamine ratio measurements were performed.
Gene expressions of HAT, HDAC1, HDAC2, H2B, H2A, H1, PRM1, PRM2, TNP1 and TNP2 were analyzed using microarray analyses.
Data were analyzed using ANOVA.
The results showed that, sperm count was significantly lower among leptin-treated rats compared to control one, whereas the fraction of sperm with abnormal morphology, the histone-to-protamine ratio and the expressions of HAT, HDAC1, HDAC2, H2B, H2A, H1, PRM1 were significantly higher in leptin treated rats.
As a conclusion, exogenous leptin administration adversely affects sperm count and morphology, which might be related to alteration in the histone-to-protamine transition during spermatogenesis.
American Psychological Association (APA)
al-Mabhuh, Fayiz A. M.& Singh, Harbindar J.. 2017. Leptin adversely affects the transition of histone-to-protamine during spermatogenesis in sprague-dawley rats. IUG Journal of Natural Studies،Vol. 25, no. 2, pp.13-20.
https://search.emarefa.net/detail/BIM-777895
Modern Language Association (MLA)
al-Mabhuh, Fayiz A. M.& Singh, Harbindar J.. Leptin adversely affects the transition of histone-to-protamine during spermatogenesis in sprague-dawley rats. IUG Journal of Natural Studies Vol. 25, no. 2 (2017), pp.13-20.
https://search.emarefa.net/detail/BIM-777895
American Medical Association (AMA)
al-Mabhuh, Fayiz A. M.& Singh, Harbindar J.. Leptin adversely affects the transition of histone-to-protamine during spermatogenesis in sprague-dawley rats. IUG Journal of Natural Studies. 2017. Vol. 25, no. 2, pp.13-20.
https://search.emarefa.net/detail/BIM-777895
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 18-20
Record ID
BIM-777895