Detection of staphylococcus aureus classic enterotoxin genes in some meat products using multiplex pcr.

Other Title(s)

تحديد جينات السموم المعوية لميكروب المكور العنقودي الذهبي في بعض منتجات اللحوم باستخدام تفاعل البلمرة المتعدد

Joint Authors

Mustafa, N. Y.
Dib, Azzah M. M.
Hammudah, Siham N.
Abd al-Bari, Shayma M.

Source

Kafr El-Sheikh Veterinary Medical Journal

Publisher

Kafr El-Sheikh University Faculty of Veterinary Medicine

Publication Date

2016-06-30

Country of Publication

Egypt

No. of Pages

12

Main Subjects

Veterinary Medicine

English Abstract

A total of 200 meat product samples of beef burger, sausage, minced meat and luncheon were randomly collected from different shops and markets in El-Gharbia and Kafr-El-Sheikh governorates.

The samples from 3 brands A, B and C were examined for the presence S.

aureus using classical bacteriological and molecular methods.

The obtained results revealed that the 2, 1 and 4 samples of beef burger, sausage and minced meat respectively, were positive for S.

aureus by PCR.

Additionally multiplex PCR was used for detection of five S.

aureus virulence classic enterotoxin genes (sea, seb, sec, sed and see).

The obtained results revealed that the beef burger samples harbored (1) seb gene; meanwhile sausage sample harbored (1) sea gene and minced meat samples harbored (2) sea, (1) sec and (1) sed genes.

This study offers the basis for further phenotypic and molecular characterization S.

aureus isolated from these products to guarantee safe consumption of meat products

Data Type

Conference Papers

Record ID

BIM-778711

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