Significant role of loss or reduced BRCA1 gene expression in clinical implication of ovarian cancer
Joint Authors
al-Budayri, Furqan J.
al-Kashwan, Dhikra A.
al-Muzaffar, Rihab H.
Source
Issue
Vol. 17, Issue 1 (30 Jun. 2014), pp.62-76, 15 p.
Publisher
University of Kufa Faculty of Medicine
Publication Date
2014-06-30
Country of Publication
Iraq
No. of Pages
15
Main Subjects
Abstract EN
Background: BRCA1 immunohistochemistry (IHC) provides a rapid initial screen to detect BRCA1 dysfunction in ovarian cancer that resulting from genetic alterations.
Aim: To assess the expression of BRCA1 protein by IHC analysis among a group of Iraqi ovarian cancer patients to evaluate the patterns of expression and its correlation with the clinicopathological parameters in attempting to evaluate a significance role of BRCA1 gene implication in ovarian cancer.
Methods: Forty three paraffin embedded samples of ovarian cancer cases were analyzed for BRCA1dysfunction by IHC analysis.
The semi-quantitative approach using modified histochemical score (H-score) was achieved to assess the patterns of BRCA1 gene expression.
Results: Complete loss of BRCA1 nuclear expression was detected in 30.2% of the cases while, reduced expression occurred in 46.5% of cases, giving rise to 76.7% of all cases detected with altered BRCA1 nuclear expression.
Altered BRCA1 expression was found to be higher in age group ≤ 45 years (78.3%) in comparison with those of ages >45 years.
Altered BRCA1 expression was significantly correlated with the high grade and with the unilateral tumor site when compared with the low grade and bilateral tumor site (P≤0.05), and was insignificantly correlated with the high stage ovarian tumors, 11.6% of cases were detected by cytoplasmic BRCA1 expression and no association was found between cytoplasmic expression and tumor grade, stage and tumor site.
Conclusion: Altered BRCA1 expression may play a significant role in the progression of ovarian cancer.
Recommendation: BRCA1 IHC is a clinically useful approach to detect the BRCA1 dysfunction and the H-score assessment reflects good estimation for BRCA1expression patterns.
American Psychological Association (APA)
al-Kashwan, Dhikra A.& al-Budayri, Furqan J.& al-Muzaffar, Rihab H.. 2014. Significant role of loss or reduced BRCA1 gene expression in clinical implication of ovarian cancer. Kufa Medical Journal،Vol. 17, no. 1, pp.62-76.
https://search.emarefa.net/detail/BIM-790716
Modern Language Association (MLA)
al-Muzaffar, Rihab H.…[et al.]. Significant role of loss or reduced BRCA1 gene expression in clinical implication of ovarian cancer. Kufa Medical Journal Vol. 17, no. 1 (2014), pp.62-76.
https://search.emarefa.net/detail/BIM-790716
American Medical Association (AMA)
al-Kashwan, Dhikra A.& al-Budayri, Furqan J.& al-Muzaffar, Rihab H.. Significant role of loss or reduced BRCA1 gene expression in clinical implication of ovarian cancer. Kufa Medical Journal. 2014. Vol. 17, no. 1, pp.62-76.
https://search.emarefa.net/detail/BIM-790716
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 73-76
Record ID
BIM-790716