عزل و تشخيص الأيشيركيا القولونية المنتجة للبيتا لاكتام الواسعة الطيف من فروج اللحم في مدينة أربيل، العراق

Abstract EN

Extended-spectrum beta-lactamase-producing Escherichia coli isolated from slaughtered broilers in retail market that sell live chickens in Erbil city, Iraq.

Forty-one cloacal fecal samples from broiler caecum were investigated from January to April 2016.

ESBLs strains were isolated using MacConkey agar supplemented with cefotaxime 1 mg/l and the isolates were identified phynotypically by biochemical tests, TBX agar and VITEK-2 compact system.

A total of 34 Escherichia coli and 4 Proteus mirabilis were analysed for determination of ESBL/AmpC by disc diffusion test using antimicrobial 68DC MAST® ESβL discs group including cefpodoxime, cefpodoxime + ESBL inhibitor, cefpodoxime + AmpC inhibitor and cefpodoxime + ESBL inhibitor + AmpC inhibitor and 67DC MAST® ESβL discs group including cefpodoxime, cefpodoxime + clavulanate, ceftazidime, ceftazidime + clavulanate, cefotaxime and cefotaxime + clavulanate.

The phenotypic results showed that in group 68DC discs 23.7% E.

coli were resistant to cefpodoxime and in group 67DC discs 73.7% of E.

coli and 7.9% of P.

mirabilis were resistance to one or more of the cefpodoxime, ceftazidime and ceftazidime.

Final results revealed that 78.0% of samples were ESBLs/ AmpC positive.

This study is the first examination to determine phenorypically E.

coli producing ESBLs/AmpC in broiler chickens in Iraq.

Conclusion, the healthy broiler can be a major source of ESBLs/AmpC and the possibility that transmitted to humans through the food chain, direct contact and the surrounding environment raises the concerns about public health and safety of poultry meat and the negative consequences of drug therapy that causes the spread of antibiotic resistance.