Detection of tetracycline resistant gen (tet K, tet M ) in some coagulase negative staphylococci isolated from different clinical sources in Erbil City
Other Title(s)
في بعض المكورات العنقودية السالبة لأنزيم التجلط (tet K ,tet M) التحري عن جينات المقاومة لمتراسايكلين المعزولة من مصادر سريرية مختلفة في مدينة أربيل
Joint Authors
Barzani, Khadijah Khalil
Abd Allah, Zayn Abd al-Ilah
Hydaeat, Soma P.
Muhammad, Asya Sadiq
Source
Journal of University of Anbar for Pure Science
Issue
Vol. 10, Issue 2 (31 Aug. 2016), pp.11-20, 10 p.
Publisher
University of Anbar College of Science
Publication Date
2016-08-31
Country of Publication
Iraq
No. of Pages
10
Main Subjects
Economics & Business Administration (Multidisciplinary)
Abstract EN
Thirty two isolates of coagulase negative staphylococci was isolated and identified from clinical sources ; including eight isolates (12.5%) of each of Staphylococcus hominis and Staphylococcus haemolyticus from (64) patients urine which suffering from urinary tract infection and eight isolates (16%) of each of Staphylococcus epidermidis and Staphylococcus auricularis from (50) ear patients which suffering from otitis in Rizgary teaching hospital in Erbil city were isolated depending on Vitek 2 compact system .
The susceptibility tests by using disc diffusion agar method were determined for all isolated coagulase negative Staphylococci to 10 different antibiotics .
From obtained results it was observed that all isolates were 100% sensitive to ceftriaxone and novobiocin, while higher resistance was obtained to tetracycline with percentage 50% and they showed differnt resistant to other remaining antibiotics.
On the other hand, all isolated Staphylococcus hominis, Staphylococcus haemolyticus , Staphylococcus epidermidis and Staphylococcus auricularis isolates were tested for tetracycline resistant genes tetK and tetM by using PCR and the results showed that 75% of Staphylococcus hominis, Staphylococcus haemolyticus and Staphylococcus epidermidis were carried tetM gene with product size about 158 bp while 50% of Staphylococcus auricularis was carried the above mentioned gen .
Also it was found that 50% of Staphylococcus hominis and Staphylococcus haemolyticus isolates in this study were harboring tetK gene with product size 360 bp and no tetK gene was observed in Staphylococcus epidermidis and Staphylococcus auricularis .
Also the antibacterial effect of two local species of menthes against resistant isolated bacteria by using the Minimum Inhibitory Concentration (MIC) method were studied .
The results showed that the MIC for aqueous extract for both plants Mentha longifolia and Mentha piperita were 1600μg/ml and it were 1400μg/ml for alcoholic extract of both plants against most Staphylococcus speciese except Staphylococcus epidermidis which the MIC was 1200 μg/ml for aqueous and 1000μg/ml for alcoholic extract of Mentha piperita, while it was 1200μg/ml for both the aqueous and alcoholic extracts for Mentha longifolia
American Psychological Association (APA)
Barzani, Khadijah Khalil& Abd Allah, Zayn Abd al-Ilah& Hydaeat, Soma P.& Muhammad, Asya Sadiq. 2016. Detection of tetracycline resistant gen (tet K, tet M ) in some coagulase negative staphylococci isolated from different clinical sources in Erbil City. Journal of University of Anbar for Pure Science،Vol. 10, no. 2, pp.11-20.
https://search.emarefa.net/detail/BIM-816178
Modern Language Association (MLA)
Barzani, Khadijah Khalil…[et al.]. Detection of tetracycline resistant gen (tet K, tet M ) in some coagulase negative staphylococci isolated from different clinical sources in Erbil City. Journal of University of Anbar for Pure Science Vol. 10, no. 2 (2016), pp.11-20.
https://search.emarefa.net/detail/BIM-816178
American Medical Association (AMA)
Barzani, Khadijah Khalil& Abd Allah, Zayn Abd al-Ilah& Hydaeat, Soma P.& Muhammad, Asya Sadiq. Detection of tetracycline resistant gen (tet K, tet M ) in some coagulase negative staphylococci isolated from different clinical sources in Erbil City. Journal of University of Anbar for Pure Science. 2016. Vol. 10, no. 2, pp.11-20.
https://search.emarefa.net/detail/BIM-816178
Data Type
Journal Articles
Language
English
Record ID
BIM-816178