MiR-96 enhances cellular proliferation and tumorigenicity of human cervical carcinoma cells through PTPN9
Joint Authors
Ma, Xiaoping
Shi, Wentian
Peng, Lina
Qin, Xuying
Hui, Yuzuo
Source
Saudi Journal of Biological Sciences
Issue
Vol. 25, Issue 5 (31 Jul. 2018), pp.863-867, 5 p.
Publisher
Publication Date
2018-07-31
Country of Publication
Saudi Arabia
No. of Pages
5
Main Subjects
Abstract EN
Up to date, the cervical cancer remains to be one of the leading gynecological malignancies worldwide.
MicroRNAs (miRNAs) play critical roles in the process of tumor initiation and progression.
However, miR-96 has rarely been investigated in human cervical carcinoma.
We aimed to investigate the biological function and underlying molecular mechanism of miR-96 in human cervical carcinoma.
MiR-96 levels were determined by qRT-PCR.
Protein tyrosine phosphatase, non-receptor type 9 (PTPN9) mRNA and protein levels were investigated by qRT-PCR and western blotting.
The cellular proliferation in cervical cells was monitored by CyQuant assay.
Soft agar assay was employed to determine the tumorigenicity.
30 UTR luciferase assay was used to validate the target gene of miR-96.
SPSS was used to analyze statistical significance in different treatment.
MiR-96 was dramatically upregulated in human cervical tumor tissues.
Overexpression of miR-96 was found to significantly promote the cellular proliferation and tumorigenicity of cervical cells.
Furthermore, we showed that PTPN9 was a direct target gene of miR-96 and had opposite effect to those of miR-96 on cervical cells.
MiR-96 may promote the cellular proliferation and tumorigenicity of cervical cells by silencing PTPN9.
Our study highlights an importantly regulatory role of miR-96 and suggests that an appropriate manipulation of miR-96 may be a new treatment of human cervical carcinoma in the future.
American Psychological Association (APA)
Ma, Xiaoping& Shi, Wentian& Peng, Lina& Qin, Xuying& Hui, Yuzuo. 2018. MiR-96 enhances cellular proliferation and tumorigenicity of human cervical carcinoma cells through PTPN9. Saudi Journal of Biological Sciences،Vol. 25, no. 5, pp.863-867.
https://search.emarefa.net/detail/BIM-838474
Modern Language Association (MLA)
Shi, Wentian…[et al.]. MiR-96 enhances cellular proliferation and tumorigenicity of human cervical carcinoma cells through PTPN9. Saudi Journal of Biological Sciences Vol. 25, no. 5 (Jul. 2018), pp.863-867.
https://search.emarefa.net/detail/BIM-838474
American Medical Association (AMA)
Ma, Xiaoping& Shi, Wentian& Peng, Lina& Qin, Xuying& Hui, Yuzuo. MiR-96 enhances cellular proliferation and tumorigenicity of human cervical carcinoma cells through PTPN9. Saudi Journal of Biological Sciences. 2018. Vol. 25, no. 5, pp.863-867.
https://search.emarefa.net/detail/BIM-838474
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 867
Record ID
BIM-838474