Prevalence and antibiotic sensitivity of escherichia coli and klebsiella pneumoniae from patients and animals in Basrah Province

Abstract EN

During the period of seven months from October 2016 to May 2017, 299 samples were collected, 152(51%) human samples, of which 69 (45.4%) were from urine and 83 (54.6%) were from children suffering from diarrhea in hospitals in Al- Basra governorate.

147 (49.2%) samples were from fecal of animals, of which 82 (55.8% ) samples were from buffalo and 65(44.2%) were from cow .A total of 101 E.

coli serotype O157: isolates out of 299 were suspected E.

coli analyzed 52/101 (34.2%%) were from human 16 (10.5%) samples were from urine and 36 (23.7%) samples were from stool .

and 49/101(33.3%) were from animal 33(22.4%) samples were from buffalo and 16 (10.9%) samples were from cow .

On the other hand 68(22.7%) isolates out of 299 were suspected K.

pneumoniae analyzed 41/68 (27%)were from human 28/41( 18.4%) isolates were from urine samples and 13/41 (8.6%) isolates were from stool samples and 27/68 (18.4%) were from animal 16 (10.9%) samples were from buffalo and 11(7.5%) samples were from cow).All suspected isolates were subjected to testing biochemical.

It was found that 10 out of 101 were 4 isolates of animal faeces (2 buffalo and 2 cows).six isolates of 52 isolates (4 of the children's stool samples and 2 of the urine samples) 19.5% nonfermented sorbitol (NSFEC).The isolates were tested against 14 different antibiotics in the manner of spread of the disk to Kirby-Pour.

All isolates were resistant to at least ten antibiotics, they showed the pattern of multiple resistance to antibiotics.Therefore, all these isolates were considered to be multidrug resistant.