Using of 2,7-dihydroxynaphthalene as a novel reagent in spectrophotometric assay of chloramphenicol
Other Title(s)
استخدام 2,7 ثنائي هيدروكسيل النفثالين بوصفه كاشف جديد للتقدير الطيفي للكلورامفينيكول
Joint Authors
Jamal, Sudad R.
Uthman, Nabil S.
Source
Publisher
University of Mosul College of Science
Publication Date
2019-04-30
Country of Publication
Iraq
No. of Pages
8
Main Subjects
Topics
English Abstract
An indirect spectrophotometric method for the determination of chloramphenicol (CAP) has been suggested.
The method is based on the oxidative coupling reaction of reduced chloramphenicol (R-CAP) with 2,7- dihydroxynaphthalene (2,7-DHN) reagent in the presence of potassium dichromate as oxidizing agent to produce brown colored, stable and soluble product.
This product showed maximum absorption at 544 nm.
Beer’s law is obeyed over the range 0.5-7.5.μg.ml-1 of R – CAP and molar absorptivity of 1.069×105 l.mol-1.cm-1 and Sandell's sensitivity index of 0.0030 μg.cm-2, limit of detection (LOD), limit of quantitation (LOQ), relative error (RE%) and relative standard deviation (RSD%) have been estimated.
The method has been successfully applied to the determination of CAP in drug formulations.
Data Type
Conference Papers
Record ID
BIM-894929
American Psychological Association (APA)
Jamal, Sudad R.& Uthman, Nabil S.. 2019-04-30. Using of 2,7-dihydroxynaphthalene as a novel reagent in spectrophotometric assay of chloramphenicol. Growing Chemistry Science and its Application (3 : 2019 : Banghazi, Libya). . Vol. 28, no. 2 (Special issue) (2019), pp.56-63.Mosul Iraq : University of Mosul College of Science.
https://search.emarefa.net/detail/BIM-894929
Modern Language Association (MLA)
Jamal, Sudad R.& Uthman, Nabil S.. Using of 2,7-dihydroxynaphthalene as a novel reagent in spectrophotometric assay of chloramphenicol. . Mosul Iraq : University of Mosul College of Science. 2019-04-30.
https://search.emarefa.net/detail/BIM-894929
American Medical Association (AMA)
Jamal, Sudad R.& Uthman, Nabil S.. Using of 2,7-dihydroxynaphthalene as a novel reagent in spectrophotometric assay of chloramphenicol. . Growing Chemistry Science and its Application (3 : 2019 : Banghazi, Libya).
https://search.emarefa.net/detail/BIM-894929
