IL-8 and IL-23 levels in peripheral blood mononuclear cells of patients with cutaneous leishmaniasis caused by leishmania major : a case-control study
Joint Authors
Khuzai, Narjis
Moghaddas, Ilham
Ridai, Sayyid Abd al-Rahim
Shamsian, Sayyid al-Akbar
Source
Iranian Red Crescent Medical Journal
Issue
Vol. 21, Issue 6 (30 Jun. 2019), pp.1-6, 6 p.
Publisher
Publication Date
2019-06-30
Country of Publication
United Arab Emirates
No. of Pages
6
Main Subjects
Topics
Abstract EN
Background: Several cytokines contribute to cutaneous leishmaniasis outcomes.
IL-23 is a key cytokine in inflammation and a central cytokine in controlling Th17.
IL-8, alsoknownas a neutrophil chemotactic factor in the lesion site of cutaneous leishmaniasis, indicates disease promotion.
Objectives: This study aimed to evaluate the relationship of Leishmania major with IL-8 and IL-23 expression.
Methods: This case-control study was performed on 25 healthy individuals and 25 cutaneous leishmaniasis patients who lived in Southwestern Iran, in 2017.
Peripheral blood mononuclear cells (PBMCs) were first isolated.
Total RNA was extracted and reversetranscribed into cDNA.
The expression levels of IL-8andIL-23 were measured by the real-timePCR.
The data were statistically analyzed using SPSS version 16.
Results: Our findings indicated that the expression of IL-8 was significantly higher in the leishmaniasis group than in the control group with the median (IQR) of 0.39 (0.92) and 0.03 (0.11), respectively (P = 0.02).
The expression of IL-23 was higher in the leishmaniasis group than in the control group with the median (IQR) of 0.13 (0.43) and 0.11 (0.61), respectively (P = 0.48).
The increased IL-8 expression accompanied the location sites in face and hand and the increased number of skin lesions.
Conclusions: The expression of IL-8 in patients with cutaneous leishmaniasis is an index of increased activity of local neutrophils that can contribute to leishmaniasis survival or inflammation increase.
However, IL-23 appears to be less important in the inflammatory reaction than IL-8.
American Psychological Association (APA)
Khuzai, Narjis& Moghaddas, Ilham& Ridai, Sayyid Abd al-Rahim& Shamsian, Sayyid al-Akbar. 2019. IL-8 and IL-23 levels in peripheral blood mononuclear cells of patients with cutaneous leishmaniasis caused by leishmania major : a case-control study. Iranian Red Crescent Medical Journal،Vol. 21, no. 6, pp.1-6.
https://search.emarefa.net/detail/BIM-895925
Modern Language Association (MLA)
Khuzai, Narjis…[et al.]. IL-8 and IL-23 levels in peripheral blood mononuclear cells of patients with cutaneous leishmaniasis caused by leishmania major : a case-control study. Iranian Red Crescent Medical Journal Vol. 21, no. 6 (Jun. 2019), pp.1-6.
https://search.emarefa.net/detail/BIM-895925
American Medical Association (AMA)
Khuzai, Narjis& Moghaddas, Ilham& Ridai, Sayyid Abd al-Rahim& Shamsian, Sayyid al-Akbar. IL-8 and IL-23 levels in peripheral blood mononuclear cells of patients with cutaneous leishmaniasis caused by leishmania major : a case-control study. Iranian Red Crescent Medical Journal. 2019. Vol. 21, no. 6, pp.1-6.
https://search.emarefa.net/detail/BIM-895925
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 6
Record ID
BIM-895925