The protective effects of honey and spirulina platensis on acetic acid-induced ulcerative colitis in rats
Joint Authors
Iftikhari, Muhammad Hassan
Ridai, Nadiyah
Tanidih, Nadir
Mukhtari, Mral
Baqiri, Zahrah
Source
Iranian Red Crescent Medical Journal
Issue
Vol. 20, Issue 4 (30 Apr. 2018), pp.1-11, 11 p.
Publisher
Publication Date
2018-04-30
Country of Publication
United Arab Emirates
No. of Pages
11
Main Subjects
Abstract EN
Background : Overproduction of reactive oxygen species (ROS) causes increased lipid peroxidation, decreased intestinal epithelial barrier integrity, and ultimately mucosal disruption and ulceration.
Several studies have confirmed the antioxidant, antiinflammatory, and immunomodulatory properties of Spirulina Platensis (SP), edible blue - green algae, in various inflammatory diseases.
In addition, natural honey, a source of phenolic and flavonoid compounds, is a powerful antioxidant, which can help prevent chronic oxidative stress and subsequent inflammation.
Objectives : In this study, we examined and compared the protective effects of SP and honey on ulcerative colitis induced by acetic acid (AA) in rats.
Methods : Forty male rats were allocated into five groups (N = 8) and received pretreatment for 32 consecutive days.
The administrations were as follows: group 1 (control) and group 2 (AA-colitis group): normal saline, group 3: 1 ml honey/day, group 4: 1 ml honey/day plus 1 g/kg SP, and group 5: 1 g/kg SP.
Colitis was induced on the 30th day in groups 2 to 5.
On day 32, the clinical activity score was determined and anesthetized animals were sacrificed.
Serum interleukin-6 (IL-6), IL-1 , tumor necrosis factor- (TNF- ), glutathione peroxidase (GPx), reduced glutathione (GSH), total antioxidant capacity (TAC), superoxide dismutase (SOD), myeloperoxidase (MPO), prostaglandin E2 (PGE2), malondialdehyde (MDA), nitric oxide (NO), and colonic weight/length ratio were determined.
In addition, histopathological changes of the colon were observed microscopically.
Results : Theinflammatory markers (PGE2, MDA,NO, IL-6, IL-1 ,MPO,andTNF- ) were significantly lower in the pretreatment groups than in the AA-colitis group (P values < 0.05).
PGE2 [median (IQR)] of the honey, SP + honey, and SP groups was [0.76 (0.33)], [0.75 (0.40)], and [0.87 (0.86)], respectively, compared to the AA-colitis group [2.60 (2.23)] (P values < 0.041).
MDA values were [6.52 (3.57)], [6.09 (3.59)], and [5.85 (4.92)] vs.
[16.60 (12.03)] (P values < 0.046) and IL-1 values were [42.20 (8.2)], [41.76 (18.10)], and [42.93 (14.09)] vs.
[79.54 (40.79)] (P values < 0.044).
Also, SOD, GSH, GPx, and TAC [median (IQR)] were significantly higher in the pretreatment groups than in the AA- colitis group (P values < 0.05).
For example, TAC values of the honey, SP + honey, and SP groups were [0.164 (0.08)], [0.14 (0.05)], and [0.16 (0.10)], respectively, vs.
the AA-colitis group [0.08 (0.01)] (P values < 0.028).
Conclusions : Honey and SP are favorable foods in preventing oxidative stress and inflammatory diseases such as ulcerative colitis.
American Psychological Association (APA)
Ridai, Nadiyah& Iftikhari, Muhammad Hassan& Tanidih, Nadir& Mukhtari, Mral& Baqiri, Zahrah. 2018. The protective effects of honey and spirulina platensis on acetic acid-induced ulcerative colitis in rats. Iranian Red Crescent Medical Journal،Vol. 20, no. 4, pp.1-11.
https://search.emarefa.net/detail/BIM-897743
Modern Language Association (MLA)
Ridai, Nadiyah…[et al.]. The protective effects of honey and spirulina platensis on acetic acid-induced ulcerative colitis in rats. Iranian Red Crescent Medical Journal Vol. 20, no. 4 (Apr. 2018), pp.1-11.
https://search.emarefa.net/detail/BIM-897743
American Medical Association (AMA)
Ridai, Nadiyah& Iftikhari, Muhammad Hassan& Tanidih, Nadir& Mukhtari, Mral& Baqiri, Zahrah. The protective effects of honey and spirulina platensis on acetic acid-induced ulcerative colitis in rats. Iranian Red Crescent Medical Journal. 2018. Vol. 20, no. 4, pp.1-11.
https://search.emarefa.net/detail/BIM-897743
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 9-11
Record ID
BIM-897743